Extracellular ATP Regulates CD73 and ABCC6 Expression in HepG2 Cells

Frontiers in Molecular Biosciences
Fabio MartinelliFaustino Bisaccia

Abstract

The ATP-binding cassette sub-family C member 6 transporter (ABCC6) is an ATP dependent transporter mainly found in the basolateral plasma membrane of hepatic and kidney cells. Mutations in ABCC6 gene were associated to the Pseudoxanthoma elasticum (PXE), an autosomal recessive disease characterized by a progressive ectopic calcification of elastic fibers in dermal, ocular, and vascular tissues. It is reported that the over-expression of ABCC6 in HEK293 cells results in the cellular efflux of ATP and other nucleoside triphosphates, which in turn are rapidly converted into nucleoside monophosphates and pyrophosphate (PPi). Since PPi is an inhibitor of mineralization, it was proposed that the absence of circulating PPi in PXE patients results in the ectopic mineralization, a typical feature of PXE. In the extracellular environment, ATP is converted, not only into pyrophosphate, but also into AMP by an ectonucleosidase, which in turn is transformed into adenosine and phosphate. ABCC6 protein is thus involved in the production of extracellular adenosine and therefore it could have a role in the activation of the purinergic system. In the liver, purinergic signaling has been shown to regulate key basic cellular functions. Our previou...Continue Reading

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Citations

Jan 2, 2021·International Journal of Molecular Sciences·Jianjian SunTao P Zhong
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fabiana GiglioAngela Ostuni
Nov 2, 2020·FEBS Letters·Shana VerschuereOlivier M Vanakker
Apr 2, 2021·The Australasian Journal of Dermatology·Millicent H Osti, Laura Scardamaglia
May 1, 2021·International Journal of Molecular Sciences·Léa VeyruneNiki Christou
May 1, 2021·International Journal of Molecular Sciences·Vittorio AbruzzeseAngela Ostuni
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valentina Lo GiudiceLuigi Todaro

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BETA
transfection
PCR

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