Extracellular ATP regulates transcervical permeability by modulating two distinct paracellular pathways

The American Journal of Physiology
G I Gorodeski, J Goldfarb

Abstract

Extracellular ATP stimulates a biphasic change in transepithelial electrical resistance (RTE) across cultures of human cervical epithelial cells: an acute decrease (phase I), followed by a delayed increase in resistance (phase II). The objective of this study was to determine the contributions of changes in the lateral intercellular space resistance (RLIS) and the tight junctional resistance (RTJ) to the changes in RTE. Phase I and phase II effects were uncoupled by treatment with 1,2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid (BAPTA)-acetoxymethyl ester, which blocks the ATP-induced increases in cytosolic Ca2+ and abolishes phase I. BAPTA-loaded cells differed from control cells in that 1) phase I began when ATP was added, in contrast to a delay of 1.5-3.5 min in phase II, 2) phase I decreases in RLIS followed a simple exponential pattern, in contrast to the complex kinetics of phase II, and 3) the magnitude of phase II varied between 20 and 100% for increases of RTJ in day 2-6 cultures; the phase I decrease of 50% in RLIS was unrelated to different experimental conditions. These results indicate that phase I and phase II are induced simultaneously and independently by ATP, and they contribute to the total changes i...Continue Reading

References

Oct 1, 1992·Physiological Reviews·N A McCarty, R G O'Neil
Jan 1, 1990·Annual Review of Physiology·S Grinstein, J K Foskett
Apr 1, 1994·Differentiation; Research in Biological Diversity·G I GorodeskiR L Eckert
Jul 1, 1994·Journal of the Society for Gynecologic Investigation·G I GorodeskiM F Romero

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Citations

Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Mar 15, 2020·Purinergic Signalling·Marta Schmidt PfaffenzellerAndréia Machado Cardoso
Dec 18, 2001·American Journal of Physiology. Cell Physiology·George I Gorodeski
Dec 18, 2001·American Journal of Physiology. Cell Physiology·George I Gorodeski
Sep 9, 1998·The American Journal of Physiology·G I GorodeskiF W Abdul-Karim
Nov 26, 2013·Purinergic Signalling·Geoffrey Burnstock
Jul 26, 2005·Journal of Reproductive Immunology·Rachel KoshiGeoffrey Burnstock

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