PMID: 9523564Apr 2, 1998Paper

Extracellular calcium deprivation in astrocytes: regulation of mRNA expression and apoptosis

Journal of Neurochemistry
R ChiesaG Forloni

Abstract

Cell viability and gene expression were studied in primary astroglial cells cultured in a nominally calcium-free medium. Ca2+ deprivation reduced progressively the astrocytes' viability, starting from 12 h; the restoration of a normal Ca2+ concentration (1.8 mM) in the medium after 12-h deprivation reversed the degenerative effect within 24 h. Biochemical and morphological examinations indicated that cell death induced by Ca2+ deprivation was mediated by apoptosis. This was associated with the expression of c-fos, c-jun, and c-myc, which, with different time courses, were induced in astrocytes after Ca2+ deprivation. Furthermore, shifting to a Ca2+-free medium modified the expression of Ich-1S transcript and rapidly increased intracellular cyclic AMP, which has been implicated in the transcriptional activation of immediate-early genes. The absence of Ca2+ in the medium reduced the expression of constitutive proteins such as alpha-actin, clusterin, glial fibrillary acidic protein, amyloid precursor protein, and glucose-6-phosphate dehydrogenase. The expression of these mRNAs was reduced >50% after 8 h of Ca2+ deprivation, when the effect on cell viability was negligible. When Ca2+ deprivation was prolonged for 24 h the expressio...Continue Reading

Citations

May 12, 2001·Cell Proliferation·F MicoudC Malcus-Vocanson
Sep 23, 2009·Apoptosis : an International Journal on Programmed Cell Death·Zhiqun ZhangKevin K W Wang
Sep 8, 2011·Journal of Cellular Physiology·Wenhao Xia, Fengyi Liang
Oct 22, 2002·Neurotoxicology·Bozena GabryelHenryk I Trzeciak
Jan 21, 2014·Biochimica Et Biophysica Acta·Giovanni De MarcoAndrea Calvo
Apr 6, 2004·Progress in Neurobiology·Kazuhiro TakumaToshio Matsuda

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