Extracellular calcium increases fibroblast growth factor 2 gene expression via extracellular signal-regulated kinase 1/2 and protein kinase A signaling in mouse dental papilla cells

Journal of Applied Oral Science : Revista FOB
Sousuke KanayaEiji Nemoto

Abstract

We previously reported that elevated extracellular calcium (Ca2+) levels increase bone morphogenetic protein 2 expression in human dental pulp (hDP) cells. However, it is unknown whether extracellular Ca2+ affects the expression of other growth factors such as fibroblast growth factor 2 (FGF2). The present study aimed to examine the effect of extracellular Ca2+ on FGF2 gene expression in hDP and immortalized mouse dental papilla (mDP) cells. Cells were stimulated with 10 mM CaCl2 in the presence or absence of cell signaling inhibitors. FGF2 gene expression was assessed using real-time polymerase chain reaction. The phosphorylation status of signaling molecules was examined by Western blotting. Extracellular Ca2+ increased FGF2 gene expression in mDP and hDP cells. Gene expression of the calcium-sensing receptor and G protein-coupled receptor family C group 6 member A, both of which are extracellular Ca2+ sensors, was not detected. Ca2+-mediated Fgf2 expression was reduced by pretreatment with the protein kinase A (PKA) inhibitor H-89 or extracellular signal-regulated kinase (ERK) 1/2 inhibitor PD98059 but not by pretreatment with the protein kinase C inhibitor GF-109203X or p38 inhibitor SB203580. Extracellular Ca2+ increased P...Continue Reading

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Citations

Feb 16, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Diana Gabriela SoaresCarlos Alberto de Souza Costa
May 24, 2019·Clinical Oral Investigations·Ester Alves Ferreira BordiniDiana Gabriela Soares
Feb 6, 2021·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·H Ali, N Raslan
Jul 29, 2021·Journal of Dental Research·D G SoaresC A de Souza Costa
Sep 16, 2021·Cell Proliferation·Ranyue RenWei Xiong

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Methods Mentioned

BETA
PCR
electrophoresis
reverse transcription-PCR
ELISA

Software Mentioned

LightCycler probe design

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