Extracellular calcium regulates the adhesion and migration of osteoclasts via integrin αv β 3 /Rho A/Cytoskeleton signaling

Cell Biology International
Bilu XiangHaiyang Yu

Abstract

Integrin αv β3 is a transmembrane integrin, which can initiate osteoclasts' attachment on bones, leading to downward signaling pathways and subsequent bone resorption. Different calcium concentrations have been reported to have an influence on the activation of integrin αv β3 . To elucidate the regulatory mechanism of extracellular calcium concentrations on osteoclasts, a controlled micro flow plate (M04S) was utilized in the ONIX flow control system to observe the osteoclasts' adhesion and migration in different calcium concentration media. Fluorescent staining is conducted to show the distribution of integrin αv β3 and cytoskeleton reorganization. In addition, western blots were performed to detect the expression of integrin αv β3 and its downstream signaling pathways related to bone resorption. Also, real-time reverse-transcription polymerase chain reaction data of transcription co-activator (YAP/TAZ) and hydrolytic enzymes (the matrix metalloproteinase 9 and cathepsin K) are evaluated. Our findings suggest that osteoclasts' migration and adhesion is better promoted at 0.5 mM than 1.2 mM, which can be partly explained by the induced cytoskeleton organization via integrin αv β3 /Rho GTPase. But the activation and nuclear loca...Continue Reading

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Citations

Feb 12, 2020·Journal of Cellular and Molecular Medicine·Lingbo KongLiang Yan
Mar 25, 2019·Stem Cells International·Rosaria SantoroGiulio Pompilio
Sep 16, 2020·International Journal of Molecular Sciences·Jiro Takito, Masanori Nakamura
Dec 31, 2020·International Journal of Molecular Sciences·Hiroyuki OkadaSakae Tanaka

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