Extracellular calcium sensing receptor stimulation in human colonic epithelial cells induces intracellular calcium oscillations and proliferation inhibition.

Journal of Cellular Physiology
Osvaldo ReyEnrique Rozengurt

Abstract

The extracellular Ca(2+)-sensing receptor (CaR) is increasingly implicated in the regulation of multiple cellular functions in the gastrointestinal tract, including secretion, proliferation and differentiation of intestinal epithelial cells. However, the signaling mechanisms involved remain poorly defined. Here we examined signaling pathways activated by the CaR, including Ca(2+) oscillations, in individual human colon epithelial cells. Single cell imaging of colon-derived cells expressing the CaR, including SW-480, HT-29, and NCM-460 cells, shows that stimulation of this receptor by addition of aromatic amino acids or by an elevation of the extracellular Ca(2+) concentration promoted striking intracellular Ca(2+) oscillations. The intracellular calcium oscillations in response to extracellular Ca(2+) were of sinusoidal pattern and mediated by the phospholipase C/diacylglycerol/inositol 1,4,5-trisphosphate pathway as revealed by a biosensor that detects the accumulation of diacylglycerol in the plasma membrane. The intracellular calcium oscillations in response to aromatic amino acids were of transient type, that is, Ca(2+) spikes that returned to baseline levels, and required an intact actin cytoskeleton, a functional Rho, Fil...Continue Reading

References

Jan 16, 1989·Biochemical and Biophysical Research Communications·K AktoriesA Hall
Sep 1, 1988·American Journal of Epidemiology·M L SlatteryM H Ford
Sep 6, 1995·Journal of the National Cancer Institute·M Lipkin, H Newmark
Sep 1, 1993·The Biochemical Journal·S E WilkinsonJ S Nixon
Apr 15, 1993·Nature
Jan 1, 1995·Journal of Cellular Biochemistry. Supplement·M Lipkin, H Newmark
Jun 1, 1996·In Vitro Cellular & Developmental Biology. Animal·M P MoyerL R Tanzer
Oct 18, 1996·Cell·K W Kinzler, B Vogelstein
Mar 1, 1997·The Journal of Clinical Investigation·K Aktories
Oct 3, 1998·JAMA : the Journal of the American Medical Association·D J Ahnen, T Byers
Jan 14, 1999·The New England Journal of Medicine·J A BaronE R Greenberg
Oct 26, 1999·Cancer Causes & Control : CCC·P PietinenJ Virtamo
Feb 11, 2000·Annals of the New York Academy of Sciences·P R Holt
Feb 11, 2000·Annals of the New York Academy of Sciences·J A BaronE R Greenberg
Apr 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y SheininH S Cross
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·A D ConigraveE M Brown
Jan 12, 2001·Physiological Reviews·E M Brown, R J MacLeod
Jun 8, 2001·The Journal of Biological Chemistry·H AwataR T Miller
Mar 21, 2002·Journal of the National Cancer Institute·Kana WuEdward L Giovannucci
May 9, 2002·American Journal of Physiology. Cell Physiology·Steven H Young, Enrique Rozengurt
Aug 28, 2002·The Journal of Biological Chemistry·Juan A RosadoStewart O Sage
Oct 3, 2002·The Journal of Biological Chemistry·Steven H YoungEnrique Rozengurt
May 27, 2003·Cell Calcium·D J BeechR Flemming
Jun 20, 2003·Current Opinion in Nephrology and Hypertension·L Darryl Quarles
Aug 2, 2003·Nature Reviews. Cancer·Sergio A Lamprecht, Martin Lipkin
Jan 28, 2004·Cell·Keith Burridge, Krister Wennerberg

❮ Previous
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Citations

Jul 17, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Daniela RiccardiWenhan Chang
Jul 11, 2012·The Journal of Biological Chemistry·Bidhan C BandyopadhyayIndu S Ambudkar
May 16, 2012·The Journal of Clinical Investigation·Venkata Lokesh BattulaMichael Andreeff
Mar 29, 2014·Cell Communication and Signaling : CCS·Daniela BassoMario Plebani
Dec 3, 2013·European Journal of Pharmacology·Anne-Sophie BorowiecThierry Capiod
Oct 25, 2012·International Journal of Nephrology·Jacob Hofman-BangEwa Lewin
Dec 29, 2015·Seminars in Cell & Developmental Biology·Jennifer L OwenMansour Mohamadzadeh
Sep 30, 2010·Life Sciences·Jai Parkash, Kamlesh Asotra
Aug 27, 2014·International Journal of Cancer. Journal International Du Cancer·NaNa KeumEdward L Giovannucci
Apr 3, 2014·International Journal of Cancer. Journal International Du Cancer·Irfete S FetahuEnikő Kállay
Dec 30, 2014·Cytokine & Growth Factor Reviews·V C RussoG A Werther
May 6, 2014·Current Opinion in Genetics & Development·Kristie McCormick, George S Baillie
Dec 27, 2012·Biochimica Et Biophysica Acta·Sarah C BrennanEnikö Kallay
Oct 30, 2016·International Journal of Molecular Sciences·Chia-Hao Lin, Pung-Pung Hwang
Mar 28, 2018·International Journal of Molecular Sciences·Andrea Gerbino, Matilde Colella
Aug 18, 2018·International Journal of Cancer. Journal International Du Cancer·Elizabeth L BarryJohn A Baron
Dec 18, 2013·American Journal of Physiology. Cell Physiology·Steven H YoungEnrique Rozengurt
Nov 13, 2014·Hormone Molecular Biology and Clinical Investigation·Lori Asarian, Thomas Bächler
Nov 19, 2011·The Journal of Biological Chemistry·Osvaldo ReyEnrique Rozengurt
Sep 1, 2017·International Journal of Cancer. Journal International Du Cancer·Fatemeh Momen-HeraviXuehong Zhang
Oct 3, 2018·World Journal of Gastroenterology : WJG·Luca IamartinoMartin Schepelmann
Mar 28, 2020·JCI Insight·Marta OnopiukKai Lau
Mar 1, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Alex TuffourHaifeng Shi
Apr 4, 2021·Nutrients·Van B LuFrank Reimann
Apr 17, 2012·Clinical Biochemistry·Bryan K WardThomas Ratajczak
Jul 1, 2021·Advances in Nutrition·Jonathan D WatkinsJavier T Gonzalez

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