Extracellular cAMP activates molecular signalling pathways associated with sperm capacitation in bovines

Molecular Human Reproduction
Carlos Agustín I AlonsoS Pérez-Martínez

Abstract

Is extracellular cAMP involved in the regulation of signalling pathways in bovine sperm capacitation? Extracellular cAMP induces sperm capacitation through the activation of different signalling pathways that involve phospholipase C (PLC), PKC/ERK1-2 signalling and an increase in sperm Ca2+ levels, as well as soluble AC and cAMP/protein kinase A (PKA) signalling. In order to fertilize the oocyte, ejaculated spermatozoa must undergo a series of changes in the female reproductive tract, known as capacitation. This correlates with a number of membrane and metabolic modifications that include an increased influx of bicarbonate and Ca2+, activation of a soluble adenylyl cyclase (sAC) to produce cAMP, PKA activation, protein tyrosine phosphorylation and the development of hyperactivated motility. We previously reported that cAMP efflux by Multidrug Resistance Protein 4 (MRP4) occurs during sperm capacitation and the pharmacological blockade of this inhibits the process. Moreover, the supplementation of incubation media with cAMP abolishes the inhibition and leads to sperm capacitation, suggesting that extracellular cAMP regulates crucial signalling cascades involved in this process. Bovine sperm were selected by the wool glass column...Continue Reading

References

Jan 15, 1992·The Biochemical Journal·H BreitbartZ Naor
Jan 1, 1995·International Journal of Clinical & Laboratory Research·R Fabiani, G Ronquist
Jul 1, 1993·The Journal of Pharmacy and Pharmacology·M R ShenS N Wu
Jul 15, 1998·Human Reproduction·L R Fraser
Aug 22, 2000·Acta Physiologica Scandinavica·J Lynge, Y Hellsten
Dec 4, 2001·Journal of Reproductive Immunology·Elisabetta BaldiGianni Forti
Aug 31, 2002·Molecular Reproduction and Development·Susan A Adeoya-Osiguwa, Lynn R Fraser
Mar 1, 2003·The Journal of Biological Chemistry·Tatiana N LitvinLonny R Levin
May 2, 2003·Biology of Reproduction·Barbara ComettiMarinella Rosselli
Aug 23, 1952·Nature·C R AUSTIN
Feb 5, 2004·Journal of Andrology·Alba MinelliBertil B Fredholm
Mar 31, 2005·International Review of Cytology·Alberto DarszonCarmen Beltrán
Apr 28, 2005·Molecular Reproduction and Development·Rhona W Baxendale, Lynn R Fraser
Apr 1, 1986·Theriogenology·J J ParrishN L First
May 15, 2007·Biology of Reproduction·Nir EtkovitzHaim Breitbart
Oct 9, 2007·Biochimica Et Biophysica Acta·Eve de Lamirande, Cristián O'Flaherty
Oct 12, 2007·Physiology·Aldebaran M Hofer, Konstantinos Lefkimmiatis
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·Z Naor, H Breitbart
Dec 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·María Gracia GervasiSilvina Perez Martinez
Apr 7, 2010·The Journal of Clinical Investigation·Masahito IkawaMasaru Okabe
Feb 25, 2011·PloS One·María Gracia GervasiSilvina Perez-Martinez
May 5, 2011·Asian Journal of Andrology·Pablo E ViscontiAlberto Darszon
Sep 29, 2011·International Journal of Andrology·P PiomboniV Zara
Jan 10, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·L ZalazarA Cesari
Mar 1, 2012·The Journal of Biological Chemistry·Jessica A MorganJohn D Schuetz
Sep 25, 2012·Asian Journal of Andrology·Debby IckowiczHaim Breitbart
Aug 3, 2013·Molecular Human Reproduction·Claudia Osycka-SalutSilvina Perez-Martinez
Oct 17, 2013·The Journal of Biological Chemistry·Eva WertheimerPablo E Visconti
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock

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Citations

Jun 30, 2019·Journal of Cell Science·C A I AlonsoS Perez-Martinez
Apr 16, 2020·Physiology·Alberto DarszonClaudia L Treviño
Nov 30, 2019·Journal of Endocrinological Investigation·I ŠabovićC Foresta
Sep 26, 2020·Scientific Reports·Nicolás ChiaranteSilvina Perez-Martinez
Oct 8, 2020·International Journal of Molecular Sciences·Nailis SyifaTzu-Hua Wu
Apr 23, 2021·Cell and Tissue Research·Veena UnnikrishnanJacob C Thundathil
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Vitaly DenisenkoTatyana Kuzmina
Oct 10, 2021·Reproduction in Domestic Animals = Zuchthygiene·Diogo Ribeiro CâmaraHeinrich Bollwein
Dec 3, 2021·Zygote : the Biology of Gametes and Early Embryos·Arlet Loza-HuertaCarmen Beltrán

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