Extracellular electron shuttling mediated by soluble c-type cytochromes produced by Shewanella oneidensis MR-1

Environmental Science & Technology
Tongxu LiuFangbai Li

Abstract

How metal-reducing bacteria transfer electrons during dissimilatory energy generation under electron acceptor-limited conditions is poorly understood. Here we incubated the iron and manganese-reducing bacterium Shewanella oneidensis MR-1 without electron acceptors. Removal of soluble extracellular organic compounds (EOCs) dramatically retarded transfer of electrons to an experimental electron acceptor, Cr(VI), by MR-1. However, the return of either high MW (>3000 Da) or low MW (<3000 Da) soluble EOCs produced by MR-1 to washed cells restored Cr(VI) reduction, though Cr(VI) reduction was fastest when both size fractions were added together. Spectral and electrochemical characterization of EOCs indicated the presence of flavins and c-type cytochromes (c-Cyts). A model of the kinetics of individual elementary reactions between cells, flavins, released c-Cyts, and Cr(VI), including the direct reduction of flavins, released c-Cyts and Cr(VI) by cells, and the indirect reduction of Cr(VI) by reduced forms of flavins and released c-Cyts, was developed. Model results suggest that released c-Cyts could act as electron mediators to accelerate electron transfer from cells to Cr(VI), and the relative contribution of this pathway was higher...Continue Reading

References

May 16, 2000·Nature·D K Newman, R Kolter
Feb 10, 2004·Applied and Environmental Microbiology·Maria E HernandezDianne K Newman
Jul 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuri A GorbyJim K Fredrickson
Feb 3, 2007·Environmental Science & Technology·Rizlan Bencheikh-LatmaniBradley M Tebo
Aug 7, 2007·Applied and Environmental Microbiology·Daniel E RossMing Tien
Dec 11, 2007·Applied and Environmental Microbiology·Harald von CansteinJonathan R Lloyd
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Enrico MarsiliDaniel R Bond
Apr 17, 2008·Environmental Microbiology·J S McLeanA S Beliaev
May 24, 2008·Geobiology·Y GorbyT J Beveridge
Jun 26, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Mackenzie Firer-SherwoodSean J Elliott
Jan 22, 2009·Analytical Biochemistry·Kenneth A JohnsonThomas Blom
Jan 22, 2009·Angewandte Chemie·Ryuhei NakamuraKazuhito Hashimoto
Dec 3, 2009·Environmental Science & Technology·Michael AeschbacherRené P Schwarzenbach
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Robert S HartshorneDavid J Richardson
Apr 19, 2011·Applied and Environmental Microbiology·Sara M BelchikLiang Shi
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas A ClarkeDavid J Richardson
Jun 15, 2011·Trends in Microbiology·Lina J BirdDianne K Newman
Feb 14, 2012·Environmental Science & Technology·Rui LiR Mark Worden
Mar 1, 2012·Frontiers in Microbiology·Liang ShiJames K Fredrickson
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Akihiro OkamotoRyuhei Nakamura
Aug 1, 2009·Environmental Microbiology Reports·Liang ShiJames K Fredrickson
Aug 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sahand PirbadianMohamed Y El-Naggar
Nov 12, 2014·Environmental Microbiology·Derek R Lovley, Nikhil S Malvankar
Nov 21, 2014·Journal of the Royal Society, Interface·Marian BreuerJulea N Butt
May 3, 2016·Advances in Microbial Physiology·G F WhiteT A Clarke
Jun 28, 2016·Applied and Environmental Microbiology·Sarah E BarchingerJohn H Golbeck
Aug 31, 2016·Nature Reviews. Microbiology·Liang ShiJames K Fredrickson
Feb 22, 2018·Science Advances·Xiao DengAkihiro Okamoto

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