PMID: 9187116Jun 1, 1997Paper

Extracellular factor(s) following exposure to alpha particles can cause sister chromatid exchanges in normal human cells

Cancer Research
B E LehnertA Deshpande

Abstract

The mechanism(s) by which alpha particles like those emitted from inhaled radon and radon progeny cause their mutagenic and carcinogenic effects remains unclear. Although direct nuclear traversals by alpha particles may be involved in mediating these outcomes, increasing evidence indicates that alpha particles can cause alterations in DNA in the absence of direct "hits" to cell nuclei. Using the occurrence of excessive sister chromatid exchanges (SCEs) as an index of DNA damage in human lung fibroblasts, we investigated the hypothesis that alpha particles may induce DNA damage via the generation of extracellular factors. We have found that a relatively low dose of alpha particles indeed results in the generation of extracellular factors, which, upon transfer to unexposed normal human cells, can cause excessive SCEs to an extent equivalent to that observed when the cells are directly irradiated with the same irradiation dose. A short-lived, SCE-inducing factor(s) was generated in alpha-irradiated culture medium containing serum in the absence of cells; it was found that the activity of this factor can be promptly inhibited by superoxide dismutase. A more persistent SCE-inducing factor(s), which can survive freeze-thawing, is hea...Continue Reading

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