Extracellular H+ dynamics during oogenesis in Rhodnius prolixus ovarioles

The Journal of Experimental Biology
C S Bjornsson, E Huebner

Abstract

The spatiotemporal dynamics of transmembrane hydrogen ion (H+) fluxes during oogenesis were investigated in the telotrophic ovarioles of the insect, Rhodnius prolixus. Although Rhodnius ovarioles possess exaggerated morphological and electrical polarity between nurse cells and oocytes, little is known about H+ changes during oogenesis, despite the regulatory role played by H+ and pH in many relevant cellular processes. A number of dynamic extracellular H+ fluxes were measured along Rhodnius ovarioles, representing an oogenesis cycle, using a non-invasive, self-referencing, H+-selective probe. The interfollicular stalk separating adjacent follicles exhibited prominent H+ efflux that peaked during midvitellogenesis and declined during late vitellogenesis. H+ efflux in this region preceded stalk formation and, importantly, preceded the onset of vitellogenesis in the adjacent posterior follicle. H+ efflux was also observed over the terminal follicle, where specialized regions of the chorion were forming, and was still detected around follicle cells after ovulation, indicating that the somatic follicular epithelium produced this flux. Transmembrane H+ fluxes may drive intracellular pH changes or may stabilize pHi in response to pH-a...Continue Reading

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Citations

Mar 29, 2014·PloS One·Joana MonteiroJoaquín Rodríguez-León
Sep 17, 2013·Memórias do Instituto Oswaldo Cruz·Petter Franco EntringerKatia Calp Gondim
Aug 21, 2012·Biochimica Et Biophysica Acta·Michele Alves-Bezerra, Katia C Gondim
Jul 9, 2011·Insect Biochemistry and Molecular Biology·Marcelo N MedeirosPedro L Oliveira
Oct 6, 2007·Insect Biochemistry and Molecular Biology·Denise M D BoutsHatisaburo Masuda
Sep 30, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Hagit Peretz-SorokaFrancis Lin
Dec 8, 2007·Archives of Insect Biochemistry and Physiology·Rachel SantosKatia C Gondim

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