PMID: 7539221May 1, 1995Paper

Extracellular heterotrimeric laminin promotes differentiation in human enterocytes

The American Journal of Physiology
P H Vachon, Jean-François Beaulieu

Abstract

To investigate the role of laminin (Ln) and merosin (a Ln variant) in the modulation of intestinal epithelial cell differentiation, we have analyzed their functional expression as well as their potential influence during the differentiation process of Caco-2 cells, a human cell line unique in its property to differentiate into a mature enterocyte-like cell type in vitro. By indirect immunofluorescence and Western blotting, Caco-2 cells have been found to express the B1 and B2 chains of Ln, as well as a heavy approximately 350- to 370-kDa chain related to the human A chain, but not the M chain, of merosin. A gradual deposition of this A-like chain-containing Ln molecule has been observed as Caco-2 cells undergo differentiation. At the cellular level, a clear relationship between basal staining for the A chain and apical staining for the brush-border membrane enzyme sucrase-isomaltase (SI) has been observed. Human Ln, used as substratum, has been found to increase significantly the expression of SI and lactase in Caco-2 cells, whereas both Ln and human merosin have been shown to stimulate aminopeptidase N and alkaline phosphatase expression. Taken together, these data indicate that enterocytic differentiation-related gene express...Continue Reading

Citations

Nov 8, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Matthew A Sanders, Marc D Basson
Dec 21, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·W LiM D Basson
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Jean-René BasqueDaniel Ménard
Apr 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Caroline FrancoeurJean-François Beaulieu
Jan 20, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Amira SeltanaJean-François Beaulieu
May 15, 2001·American Journal of Physiology. Cell Physiology·R GauthierP H Vachon
Jul 17, 2008·Journal of Cell Science·Zhen X MahoneyJeffrey H Miner

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