PMID: 8947495Nov 15, 1996Paper

Extracellular high-mobility group 1 protein is essential for murine erythroleukaemia cell differentiation

The Biochemical Journal
Bianca SparatoreSandro Pontremoli

Abstract

A high-mobility group 1 (HMG1) protein type isolated from murine erythroleukaemia (MEL) cells promotes acceleration of the differentiation process when added to a MEL cell culture together with the inducer hexamethylene bisacetamide. We now provide direct evidence that the presence of HMG1 protein in the extracellular medium is essential for terminal erythroid differentiation. An extracellular function for HMG1 protein in MEL cell is further supported by a demonstration that this protein is released from MEL cells exposed to the chemical inducer and that the addition of an anti-(HMG1 protein) monoclonal antibody to the cell culture inhibits the differentiation process almost completely. The release of HMG1 protein from MEL cells is modulated by compounds affecting cell calcium homoeostasis, such as a calcium ionophore or verapamil. In fact, in the presence of the ionophore an increased rate of differentiation is accompanied by an enhanced extracellular release of HMG1 protein, whereas in the presence of verapamil both phenomena are significantly decreased.

Citations

Jul 11, 2014·Molecular Aspects of Medicine·Rui KangDaolin Tang
Aug 21, 2013·Medical Hypotheses·Xue RuiYang Jinjian
Jun 24, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Guoqian ChenHaichao Wang
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