Extracellular Matrix and Oxidative Phosphorylation: Important Role in the Regulation of Hypothalamic Function by Gut Microbiota

Frontiers in Genetics
Xunzhong QiPeng Xie

Abstract

In previous studies, our team examined the gut microbiota of healthy individuals and depressed patients using fecal microbiota transplantation of germ-free (GF) mice. Our results showed that depression-like and anxiety-like behavioral phenotypes of host mice were increased, but the molecular mechanism by which gut microbiota regulate host behavioral phenotypes is still unclear. To investigate the molecular mechanism by which gut microbiota regulate host brain function, adult GF mice were colonized with fecal samples derived from healthy control (HC) individuals or patients with major depressive disorder (MDD). Transcriptomic profiling of hypothalamus samples was performed to detect differentially expressed genes (DEGs). qRT-PCR was used for validation experiments. Colonization germ-free (CGF) mice had 243 DEGs compared with GF mice. The most enriched KEGG pathways associated with upregulated genes were "protein digestion and absorption," "extracellular matrix (ECM)-receptor interaction," and "focal adhesion." MDD mice had 642 DEGs compared with HC mice. The most enriched KEGG pathways associated with upregulated genes in MDD mice were also "protein digestion and absorption," "ECM-receptor interaction," and "focal adhesion." Mea...Continue Reading

References

May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Manuel E ThanWolfram Bode
Mar 18, 2003·Journal of Clinical Psychopharmacology·Thomas NickelFlorian Holsboer
Jul 16, 2003·Biochemical and Biophysical Research Communications·Christopher S ChenDonald E Ingber
Mar 6, 2004·Trends in Microbiology·Lora V Hooper
May 26, 2004·Biochemical Society Transactions·R Zaidel-BarB Geiger
Feb 3, 2005·Molecular Psychiatry·A Marques-DeakE Sternberg
Mar 26, 2005·Science·Fredrik BäckhedJeffrey I Gordon
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuanyi FengChristopher A Walsh
Apr 5, 2007·Nature Protocols·Robert M J Deacon
Nov 13, 2009·Investigative Ophthalmology & Visual Science·Germán Pinzón-DuarteWilliam J Brunken
Dec 31, 2009·Journal of Lipid Research·Vidya R VelagapudiFredrik Bäckhed
Jun 23, 2010·Human Molecular Genetics·Johannes A MayrJosef Houstek
Aug 18, 2010·The Journal of Experimental Medicine·Hervé FaletJohn H Hartwig
Sep 8, 2010·Trends in Cell Biology·Monika Abedin, Nicole King
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rochellys Diaz HeijtzSven Pettersson
Jul 23, 2011·Science·Rosa C PaolicelliCornelius T Gross
Aug 30, 2011·Briefings in Bioinformatics·Pascale GaudetPaul D Thomas
Jan 14, 2012·Journal of Proteome Research·Peng ZhengPeng Xie
Feb 9, 2012·Molecular & Cellular Proteomics : MCP·Kim HenningsenOve Wiborg
Apr 14, 2012·Journal of Medical Genetics·Tobias B HaackHolger Prokisch
May 5, 2012·Nature Protocols·David A Slattery, John F Cryan
Jun 8, 2012·Science·Jeremy K NicholsonSven Pettersson
Jun 8, 2012·Science·Lora V HooperAndrew J Macpherson
Nov 1, 2012·Molecular & Cellular Proteomics : MCP·Peng ZhengPeng Xie
Aug 24, 2013·Circulation Research·Nobel C ZongPeipei Ping
Oct 29, 2013·Molecular and Cellular Neurosciences·Lauren SummersEmmanuel Pinteaux
Feb 8, 2014·Journal of Proteomics·Leonid TarassishinSunhee C Lee
Nov 21, 2014·Science Translational Medicine·Viorica BranisteSven Pettersson
Nov 22, 2014·Nature Reviews. Molecular Cell Biology·Caroline BonnansZena Werb
Jun 2, 2015·Nature Neuroscience·Daniel ErnyMarco Prinz
Jun 17, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Luis Mauricio Hurtado-LópezNorma Estela Herrera González
Nov 13, 2015·Advanced Drug Delivery Reviews·Achilleas D TheocharisNikos K Karamanos

❮ Previous
Next ❯

Citations

May 18, 2021·The Journal of Clinical Investigation·Kristie B Yu, Elaine Y Hsiao
Jul 6, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xinyi GuMingmei Zhou

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA637458

Methods Mentioned

BETA
PCR
RNA-seq
gastric bypass

Software Mentioned

Search Tool for the Retrieval of Interacting Genes ( STRING
MHupM
SMART

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Bacteriotherapy (ASM)

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.

Bacteriotherapy

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.