Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix

Acta Biomaterialia
Majed FelembanMajlinda Lako

Abstract

The extracellular matrix (ECM) plays an important role in numerous processes including cellular proliferation, differentiation, migration, maturation, adhesion guidance and axonal growth. To date, there has been no detailed analysis of the ECM distribution during retinal ontogenesis in humans and the functional importance of many ECM components is poorly understood. In this study, the expression of key ECM components in adult mouse and monkey retina, developing and adult human retina and retinal organoids derived from human pluripotent stem cells was studied. Our data indicate that basement membrane ECMs (Fibronectin and Collagen IV) were expressed in Bruch's membrane and the inner limiting membrane of the developing human retina, whilst the hyalectins (Versican and Brevican), cluster of differentiation 44 (CD44), photoreceptor-specific ECMs Interphotoreceptor Matrix Proteoglycan 1 (IMPG1) and Interphotoreceptor Matrix Proteoglycan 2 (IMPG2) were detected in the developing interphotoreceptor matrix (IPM). The expression of IMPG1, Versican and Brevican in the developing IPM was conserved between human developing retina and human pluripotent stem cell-derived retinal organoids. Blocking the action of CD44 and IMPG1 in pluripotent...Continue Reading

Citations

Mar 29, 2019·Scientific Reports·Tsunehiko IkedaShinji Takai
Jun 21, 2020·International Journal of Molecular Sciences·Jacqueline ReinhardVinodh Kakkassery
Aug 25, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Li ChenQuan Wei
May 12, 2020·Tissue Engineering and Regenerative Medicine·Sanaz BehtajMaksym Rybachuk
Aug 9, 2020·Stem Cells Translational Medicine·Ali E GhareebDavid H Steel
Dec 5, 2019·Genes·Peter M J Quinn, Jan Wijnholds
Aug 16, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Arwin ShresthaKristan S Worthington
Nov 3, 2020·Frontiers in Cellular Neuroscience·Ratnesh K Singh, Igor O Nasonkin
Dec 29, 2020·Development·Michelle O'Hara-Wright, Anai Gonzalez-Cordero
Jul 27, 2021·Development·Michelle O'Hara-Wright, Anai Gonzalez-Cordero

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