PMID: 7514938Jan 1, 1994Paper

Extracellular matrix-dependent differentiation of rabbit tracheal epithelial cells in primary culture

In Vitro Cellular & Developmental Biology. Animal
A Baeza-SquibanF Marano

Abstract

The differentiation of tracheal epithelial cells in primary culture was investigated according to the nature of the extracellular matrix used. Cultures obtained by the explant technique were realized on a type I collagen substratum either as a thin, dried coating or as a thick, hydrated gel supplemented with culture medium and serum. These two types of substratum induced distinct cell morphology and cytokeratin expression in the explant derived cells. Where cells are less proliferating (from Day 7 to 10 of culture), differentiation was evaluated by morphologic ultrastructural observations, immunocytochemical detection of cytokeratins, and determination of cytokeratin pattern by biochemical analysis. The epithelium obtained on gel was multilayered, with small, round basal cells under large, flattened upper cells. The determination of the keratin pattern expressed by cells grown on gel revealed an expression of keratin 13, already considered as a specific marker of squamous metaplasia, that diminished with retinoic acid treatment. Present results demonstrated by confocal microscopy that K13-positive cells were large upper cells with a dense keratin network, whereas lower cells were positively stained with a specific monoclonal an...Continue Reading

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Citations

May 4, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Baeza-SquibanF Marano
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S BolandF Marano
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