Extracellular Matrix Glycoprotein-Derived Synthetic Peptides Differentially Modulate Glioma and Sarcoma Cell Migration

Cellular and Molecular Neurobiology
Nicole BrösickeAndreas Faissner

Abstract

Glycoproteins of the extracellular matrix (ECM) regulate proliferation, migration, and differentiation in numerous cell lineages. ECM functions are initiated by small peptide sequences embedded in large constituents that are recognized by specific cellular receptors. In this study, we have investigated the biological effects of peptides derived from collagen type IV and tenascin-C compared to the well-known RGD peptide originally discovered in fibronectin. The influence of glycoproteins and corresponding peptides on the migration of the glioma cell lines U-251-MG and U-373-MG and the sarcoma line S-117 was studied. When the cell lines were tested in a modified Boyden chamber assay on filters coated with the ECM glycoproteins, glioma cells showed a strong migration response on tenascin-C and the basal lamina constituent collagen IV, in contrast to S-117 cells. In order to identify relevant stimulatory motifs, peptides derived from fibronectin (6NHX-GRGDSF), tenascin-C (TN-C, VSWRAPTA), and collagen type IV (MNYYSNS) were compared, either applied in solution in combination with ECM glycoprotein substrates, in solution in the presence of untreated membranes, or coated on the filters of the Boyden chambers. Using this strategy, we ...Continue Reading

References

Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·E RuoslahtiW A Border
Sep 1, 1990·The British Journal of Dermatology·C G SchirrenO Braun-Falco
Mar 1, 1990·Journal of Neurochemistry·A FaissnerM Schachner
May 6, 1988·Cell·R Chiquet-EhrismannM Chiquet
Jan 1, 1987·Annual Review of Cell Biology·C A Buck, A F Horwitz
Jun 1, 1986·Scientific American·R O Hynes
Jan 1, 1968·Acta Pathologica Et Microbiologica Scandinavica·J Pontén, E H Macintyre
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M D Pierschbacher, E Ruoslahti
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M PierschbacherE Ruoslahti
Oct 10, 1997·Cell and Tissue Research·A Faissner
Jul 14, 2000·Journal of Neuro-oncology·S ChatterjeeG Y Gillespie
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·H L Hadden, C A Henke
Jul 8, 2003·The Journal of Pathology·Ruth Chiquet-Ehrismann, Matthias Chiquet
May 5, 2004·The Journal of Biological Chemistry·Kenneth C InghamHarold P Erickson
Apr 8, 2006·The EMBO Journal·Matthew J HayesStephen E Moss
May 26, 2006·The American Journal of Pathology·Gui SuAndreas Friedl
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Muhammad H ZamanPaul Matsudaira
Aug 8, 2006·Journal of Molecular Biology·Shalini IyerK Ravi Acharya
Sep 7, 2007·Acta Neuropathologica·An ClaesPieter Wesseling
Feb 5, 2008·The International Journal of Biochemistry & Cell Biology·Tracy L Adair-Kirk, Robert M Senior
May 20, 2008·The International Journal of Biochemistry & Cell Biology·Daniel C Worth, Maddy Parsons
Aug 2, 2008·The New England Journal of Medicine·Patrick Y Wen, Santosh Kesari
Dec 26, 2008·Molecular Biology of the Cell·Selwyn A Williams, Jean E Schwarzbauer
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michèle C EratIoannis Vakonakis
Aug 21, 2009·Cell and Tissue Research·Malgorzata BarczykDonald Gullberg
Oct 20, 2009·Journal of Cell Communication and Signaling·Kim S Midwood, Gertraud Orend
Oct 27, 2009·Developmental Biology·Tania Rozario, Douglas W DeSimone
Mar 10, 2010·Archives of Neurology·Louis B NaborsHassan M Fathallah-Shaykh
Aug 17, 2010·European Journal of Cell Biology·Luis Castro-SanchezEduardo Perez Salazar
Apr 6, 2011·Journal of the National Comprehensive Cancer Network : JNCCN·David A ReardonHenry S Friedman
Aug 6, 2011·Cellular and Molecular Life Sciences : CMLS·Kim S MidwoodGertraud Orend
Apr 13, 2012·Anti-cancer Drugs·Elena Victoria RoscaJohn Laterra
Nov 13, 2012·Acta Neuropathologica·Karl H PlateDaniel J Dumont
May 9, 2013·The Journal of Biological Chemistry·Michèle C EratIoannis Vakonakis
May 25, 2013·The Journal of Biological Chemistry·Gangli LiuMaria A Kukuruzinska

❮ Previous
Next ❯

Citations

Nov 7, 2016·Cellular and Molecular Neurobiology·Jianguo ChenQingfeng Huang
Oct 6, 2018·Seminars in Cell & Developmental Biology·Lars Roll, Andreas Faissner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.