PMID: 3768884Aug 15, 1986Paper

Extracellular matrix proteoglycans and cell-substratum adhesion of human endothelial cells: the effect of methyl beta-D-xylopyranoside

Carbohydrate Research
P B GordonV B Hatcher

Abstract

The influence of methyl beta-D-xylopyranoside on human endothelial cell proteoglycans isolated from the medium and extracellular matrix was investigated. Confluent cultures of human endothelial cells incorporate significant amounts of heparan sulfate (78%), chondroitin sulfate (10%), and dermatan sulfate (12%) into the extracellular matrix. Chondroitin sulfate (35%) and dermatan sulfate (37%) were the major glycosaminoglycans present in the medium. In the presence of methyl beta-D-xylopyranoside, incorporation of labeled proteoglycans into extracellular matrix was diminished by approximately 70%. Heparan sulfate comprised the major proteoglycan present in extracellular matrix (89%) in cells grown in the presence of methyl beta-D-xylopyranoside. In contrast to the incorporation of proteoglycan into extracellular matrix, methyl beta-D-xylopyranoside stimulated the secretion of labeled glycosaminoglycan chains into the medium 2.5-fold. In the presence of methyl beta-D-xylopyranoside, secretion of chondroitin sulfate into the medium was markedly stimulated, with a slight increase in secretion of heparan sulfate. Chondroitin sulfate (62%) and heparan sulfate (34%) were the major labeled glycosaminoglycans present in medium from meth...Continue Reading

References

Jan 1, 1979·Journal of Supramolecular Structure·L A CulpB J Rollins
Jan 1, 1979·Archives of Biochemistry and Biophysics·S LohmanderA Hinek
Dec 1, 1975·Biochemical and Biophysical Research Communications·N B Schwartz, A Dorfman
Dec 1, 1984·Journal of Cellular Physiology·P B GordonV B Hatcher
Dec 1, 1983·Journal of Cellular Physiology·J Robinson, D Gospodarowicz
Aug 1, 1984·Journal of Cellular Physiology·R HaasL A Culp
Sep 1, 1983·In Vitro·P B GordonV B Hatcher
Aug 1, 1984·The Journal of Cell Biology·Y S KanwarJ T Gibbons
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·A Heifetz, A R Johnson

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Citations

Jul 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A M Schor, S L Schor
Jul 1, 1995·In Vitro Cellular & Developmental Biology. Animal·M PiepkornA Linker
Aug 1, 1988·Developmental Biology·B LelongtY S Kanwar
Sep 30, 1991·Biochemical and Biophysical Research Communications·M PiepkornA Linker

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