Extracellular matrix regulates the amount of the beta-amyloid precursor protein and its amyloidogenic fragments

Journal of Cellular Physiology
F C BronfmanN C Inestrosa

Abstract

We have studied the influence of the extracellular matrix (ECM) on the amount of beta-amyloid precursor protein (APP) and C-terminal amyloid-bearing fragments in 313 fibroblasts. After incubation with ECM components, the cellular APP content of 3T3 cells changed. Besides, different substrata including collagen, fibronectin, laminin, vitronectin, and heparin, determined changes in the amount of a C-terminal 22 kDa-fragment. The regulation of amyloidogenic fragments by the ECM was transient; in fact, when 3T3 cells were plated on tissue culture dishes coated with collagen or vitronectin, maximal levels of the 22 kDa fragment were observed 12 h after plating; in the presence of fibronectin, the maximum level of the amyloidogenic fragment was obtained 36 h after plating. These results indicate that the ECM modulates in a transient way the generation of APP-derived polypeptides containing the amyloid-beta-peptide (A beta). The ECM does not have a generalized effect on 3T3 fibroblasts, because no significant differences in cell attachment, growth rate, whole-cell polypeptide pattern beta 1 integrin and alpha-tubulin levels were observed on cells grown on various matrix proteins. Laminin, collagen, and heparin also influence the level...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A TamaokaD J Selkoe
Jan 1, 1991·Journal of Neuroscience Research·K C BreenB H Anderton
Jan 1, 1991·Annual Review of Neuroscience·L F Reichardt, K J Tomaselli
May 7, 1990·Brain Research·F G KlierD Schubert
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D J SelkoeT Oltersdorf
Oct 1, 1986·Annals of Neurology·B T HymanA R Damasio
Nov 1, 1982·The Journal of Cell Biology·R O Hynes, K M Yamada
Oct 1, 1993·Trends in Neurosciences·M Mullan, F Crawford
Mar 31, 1995·The Journal of Biological Chemistry·U MönningK Beyreuther
Apr 14, 1995·Science·E A Clark, J S Brugge
Mar 1, 1995·The Journal of Cell Biology·B AllinquantA Prochiantz
Oct 1, 1994·Journal of Neurochemistry·C SotoN C Inestrosa
Jan 1, 1993·American Indian and Alaska Native Mental Health Research : the Journal of the National Center·G P Sage, G L Burns
Jan 1, 1994·Annual Review of Neuroscience·D J Selkoe
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M C KibbeyH K Kleinman
Sep 1, 1993·General Pharmacology·E Brandan, N C Inestrosa
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C Q Lin, M J Bissell

❮ Previous
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Citations

May 21, 1998·Brain Research·A MonjiN Tashiro
Sep 1, 2000·Brain Research. Brain Research Reviews·G V De Ferrari, N C Inestrosa
Nov 8, 1996·Neuroscience Letters·F C BronfmanN C Inestrosa
Apr 27, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C Morgan, N C Inestrosa
Sep 12, 2006·Neurochemistry International·Annamária BjelikJános Kálmán
Jun 24, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Martin WinterChristoph Röcken
Oct 31, 2000·Microscopy Research and Technique·K A McGowan, M P Marinkovich
Jul 12, 2002·Journal of Neuroscience Research·Edouard Palu, Päivi Liesi
Oct 23, 2002·Journal of Neuroscience Research·Bruce Teter, J Wesson Ashford
Jun 1, 2012·Molecular Neurobiology·M T GentileL Colucci-D'Amato
Oct 28, 2016·Journal of Biomechanical Engineering·Eric S HaldPatrick W Alford
Jul 28, 2020·International Journal of Molecular Sciences·Álvaro Ruiz-AriasAngel Orte
Aug 26, 1999·Journal of Neurochemistry·F H RuizN C Inestrosa
Apr 2, 2019·Frontiers in Cell and Developmental Biology·Ewa E Bres, Andreas Faissner
Apr 18, 2021·Scientific Reports·Francesca GenovaMaria Longeri

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