Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study

BMC Veterinary Research
Manuela MartanoPaola Maiolino

Abstract

Equine sarcoids are locally invasive, fibroblastic benign skin tumors. Bovine papillomavirus type-1 (BPV-1) and/or Bovine papillomavirus type-2 (BPV-2) are believed to be the causative agent of sarcoids, although the mechanisms by which the virus induce the tumor are still poorly understood. We hypothesized that in genetically predisposed equines latent BPV infection may be reactivated by immunosoppression and/or mechanical injury leading to a form of pathologic wound which may transform into a sarcoid. In this study, we investigated in 25 equine sarcoids and in five normal skin samples the histological features and evaluated the immunohistochemical and molecular expression of type I and type III Collagen, vimentin (VIM), alfa Smooth Muscle Actin (α-SMA), Matrix Metalloproteinase (MMPs) -2, 9, 14 and tissue inhibitor of metalloproteinase 2 (TIMP-2). In 64% of investigated sarcoids, type I collagen staining was stronger than that of type III collagen. In 80% of sarcoids, SFs were strongly positive for vimentin and negative for α-SMA; the remaining sarcoid samples (20%) showed 70-80% of SFs labeled for vim and approximately 20-30% labeled for α-SMA. Moreover, all sarcoid specimen showed a variable staining pattern (weak to modera...Continue Reading

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Citations

Feb 18, 2017·Genetics and Molecular Biology·Rodrigo Pinheiro AraldiWilly Beçak
Jul 13, 2020·Equine Veterinary Journal·Ewelina Semik-Gurgul
Sep 5, 2018·BMC Veterinary Research·Manuela MartanoPaola Maiolino
Jan 20, 2021·Veterinary Pathology·Magdalena OgłuszkaAndrzej Raś

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Methods Mentioned

BETA
zymography
biopsy
light microscopy
protein assay
electrophoresis

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