Extracellular matrix synthesized by clonal osteogenic cells is osteoinductive in vivo and in vitro: role of transforming growth factor-beta 1 in osteoblast cell-matrix interaction

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
M Kirk, A J Kahn

Abstract

ROB-C26 (C26) is a multipotential, clonal cell line known to express several members of the TGF-beta superfamily and to become more osteoblastic (e.g., express higher levels of alkaline phosphatase) upon treatment with 10(-6)M retinoic acid (RA). We hypothesize that the expression of this more osteoblastic phenotype subsequent to RA exposure is the result of the treated cell's extracellular matrix (ECM) becoming a repository and active source of putative osteoinductive growth factors including, specifically, select members of the TGF-beta superfamily. To test this hypothesis, we isolated the ECM from RA-treated and untreated C26 cells and assessed them for their ability to promote osteogenic differentiation in vivo and in vitro. We then explored whether the latter activities could be attributed specifically to TGF-beta 1. We found that the ECM of treated cells isolated by cell lysis and extensive washing induced endochondral bone formation in vivo when implanted into the thigh muscles of athymic nude mice and stimulated alkaline phosphatase (ALP) activity in vitro in freshly plated C26 cells. This latter stimulation was comparable to levels observed with direct RA treatment. This latter in vitro activity was only very partially...Continue Reading

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Citations

Mar 22, 2005·Toxicon : Official Journal of the International Society on Toxinology·Rong-Sen YangWen-Mei Fu
Jul 25, 2015·International Journal of Oral Science·Dian JingZhi-He Zhao
Apr 20, 2018·Journal of Cardiovascular Development and Disease·Froso SophocleousGiovanni Biglino
Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S ShiA J Kahn
Dec 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K K PunP W Ho
Dec 24, 2015·Stem Cell Research & Therapy·Catherine M KolfRocky S Tuan
Nov 27, 2014·Journal of Cellular Biochemistry·Abdul S QadirJeong-Hwa Baek
Jul 17, 2001·Journal of Periodontology·L MarinucciP Locci
Dec 17, 1998·Journal of Biomedical Materials Research·J H Brekke, J M Toth
Apr 20, 2002·The Journal of Biological Chemistry·Richard C D'AlonzoNicola C Partridge
Jan 22, 1998·Biochemical and Biophysical Research Communications·C FaucheuxJ Amédée
Jan 29, 2005·The Journal of Investigative Dermatology·Jae-Bum JunKeith B Elkon

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