Extracellular methylglyoxal toxicity in Saccharomyces cerevisiae: role of glucose and phosphate ions

Journal of Applied Microbiology
K IspolnovAna Ponces Freire

Abstract

The purpose of this study was to investigate the behaviour of Saccharomyces cerevisiae in response to extracellular methylglyoxal. Cell survival to methylglyoxal and the importance of phosphates was investigated. The role of methylglyoxal detoxification systems and methylglyoxal-derived protein glycation were studied and the relation to cell survival or death was evaluated. Extracellular methylglyoxal decreased cell viability, and the presence of phosphate enhanced this effect. D-glucose seems to exert a protective effect towards this toxicity. Methylglyoxal-induced cell death was not apoptotic and was not related to intracellular glycation processes. The glyoxalases and aldose reductase were important in methylglyoxal detoxification. Mutants lacking glyoxalase I and II showed increased sensitivity to methylglyoxal, while strains overexpressing these genes had increased resistance. Extracellular methylglyoxal induced non-apoptotic cell death, being unrelated to glycation. Inactivation of methylglyoxal-detoxifying enzymes by phosphate is one probable cause. Phosphate and D-glucose may also act through their complex involvement in stress response mechanisms. These findings contribute to elucidate the mechanisms of cell toxicity b...Continue Reading

References

Apr 1, 1978·Analytical Biochemistry·M W KellumS J Norton
Dec 1, 1992·The Journal of Clinical Investigation·N Ueda, S V Shah
Jul 20, 1995·Analytical Biochemistry·C Cordeiro, A P Freire
Aug 1, 1997·Archives of Biochemistry and Biophysics·I N ShipanovaR H Nagaraj
Apr 18, 1998·The Journal of Biological Chemistry·F A ShamsiR H Nagaraj
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·F W ChaplenD C Cameron
Jan 19, 1999·Diabetes·P J BeisswengerB S Szwergold
Jun 8, 2001·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C JacksonP Sambrook
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Franky Van HerrewegheKatia Vancompernolle
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Dec 4, 2003·Biochemical Society Transactions·A Ponces FreireC Cordeiro
Mar 26, 2004·FEMS Yeast Research·Jaime Aguilera, Jose Antonio Prieto
Jan 18, 2005·Current Opinion in Immunology·Dennis H Kim, Frederick M Ausubel

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Citations

Mar 29, 2016·Biochemistry Research International·Bohdana V ValishkevychHalyna M Semchyshyn
Apr 2, 2020·TheScientificWorldJournal·Halyna Semchyshyn

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