Extracellular Mg2+ inhibits capacitative Ca2+ entry in vascular smooth muscle cells

Circulation
M YoshimuraG Kajiyama

Abstract

Agonist-induced Ca2+ entry is thought to be mediated by capacitative Ca2+ entry other than L-type Ca2+ channels in vascular smooth muscle cells (VSMCs). The mechanism for capacitative Ca2+ entry has not been fully elucidated. Our objective was to examine the effect of external Mg2+ on capacitative Ca2+ entry in cultured rat aortic VSMCs. Three doses of external Mg2+ concentration (nominally 0, 1, and 5 mmol/L) were used. After exposure to 1 mumol/L, angiotensin II (Ang II) in Ca(2+)-free medium, addition of Ca2+ to the medium caused an increase in cytosolic free Ca2+ concentration ([Ca2+]i), indicating Ang II-induced Ca2+ influx. This Ca2+ influx was attenuated in cells preincubated with high external Mg2+ concentrations or with 1 mumol/L nifedipine. After VSMCs in Ca(2+)-free medium were exposed to 1 mumol/L thapsigargin, which inhibits the sarcoplasmic reticulum Ca(2+)-ATPase and depletes Ca2+ stores, addition of Ca2+ to the medium induced an increase in [Ca2+]i, indicating capacitative Ca2+ entry. This entry pathway was found to be independent of dihydropyridine-sensitive Ca2+ channels and inhibited by increased external Mg2+ concentration. External Mg2+ concentration did not influence Ca2+ efflux across the plasma membrane ...Continue Reading

References

Jun 1, 1992·The Journal of Clinical Investigation·E K D'AngeloC M Rembold
Dec 1, 1991·Hypertension·T OshimaD A McCarron
Jan 1, 1991·Annual Review of Physiology·P W Flatman
Mar 30, 1990·Biochemical and Biophysical Research Communications·G A Quamme, S W Rabkin
Jan 1, 1989·Annual Review of Physiology·C van Breemen, K Saida
Mar 1, 1989·The American Journal of Physiology·B RajuR E London
Feb 1, 1982·The Journal of Cell Biology·S GuntherM A Gimbrone
Jul 1, 1995·Journal of Vascular Research·B HimpensR Casteels
Jan 1, 1993·Progress in Cardiovascular Diseases·M A Arsenian
Nov 1, 1994·Pflügers Archiv : European journal of physiology·C Van Renterghem, M Lazdunski
Jan 1, 1995·Journal of Molecular and Cellular Cardiology·Z Xiong, N Sperelakis
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Nov 1, 1994·The American Journal of Physiology·T RohácsA Spät
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A D ShortD L Gill
Oct 22, 1993·Cell·J W Putney, G S Bird

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Citations

Jan 23, 2003·Molecular Aspects of Medicine·R M Touyz
Jul 14, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Fatih KircelliJutta Passlick-Deetjen
Dec 27, 2005·Circulation Journal : Official Journal of the Japanese Circulation Society·Hangyuan GuoTakanori Ueda
Dec 15, 2010·Circulation Journal : Official Journal of the Japanese Circulation Society·Alvaro YogiRhian M Touyz
Jun 5, 2014·Cardiology in Review·Dhaval KolteWilliam H Frishman
May 28, 2010·Journal of Perinatology : Official Journal of the California Perinatal Association·Y KatayamaY K Lee
Jul 17, 1998·Anesthesiology·M N Gomez
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Zhen ZhenYufeng Zheng
Dec 25, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Zhan AverbukhJoshua Weissgarten
Jun 10, 2006·Archives of Biochemistry and Biophysics·Bruno Sontia, Rhian M Touyz
Mar 19, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Hyun Soo ParkInsuk So
Dec 30, 2016·Journal of Hypertension·Yosuke FunatoHiroaki Miki
Dec 17, 2010·World Journal of Cardiology·Ming-Jui Hung
Jan 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Rhian M Touyz
Apr 30, 1999·Physiological Reviews·M J Davis, M A Hill
Sep 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jin ZhangDonald R Matteson
Jan 25, 2000·American Journal of Physiology. Cell Physiology·A ArnonM P Blaustein
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A ArnonM P Blaustein
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S S McDanielJ X Yuan
Jul 28, 2020·International Journal of Molecular Sciences·Philipp GlobigBérengère J C Luthringer-Feyerabend
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Jin ZhangMordecai P Blaustein
Sep 20, 2000·American Journal of Physiology. Heart and Circulatory Physiology·O Stepien, P Marche
Jul 12, 2018·International Journal of Hypertension·Andrea M P Romani
Jan 26, 2020·BMJ Evidence-based Medicine·Charat ThongprayoonKianoush B Kashani
Oct 12, 1999·The American Journal of Physiology·S K Fellner, W J Arendshorst
May 18, 2021·Postgraduate Medicine·Charat ThongprayoonWisit Cheungpasitporn

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