Extracellular microRNAs in blood differentiate between ischaemic and haemorrhagic stroke subtypes

Journal of Extracellular Vesicles
M Yashar S KalaniKendall Van Keuren-Jensen

Abstract

Rapid identification of patients suffering from cerebral ischaemia, while excluding intracerebral haemorrhage, can assist with patient triage and expand patient access to chemical and mechanical revascularization. We sought to identify blood-based, extracellular microRNAs 15 (ex-miRNAs) derived from extracellular vesicles associated with major stroke subtypes using clinical samples from subjects with spontaneous intraparenchymal haemorrhage (IPH), aneurysmal subarachnoid haemorrhage (SAH) and ischaemic stroke due to cerebral vessel occlusion. We collected blood from patients presenting with IPH (n = 19), SAH (n = 17) and ischaemic stroke (n = 21). We isolated extracellular vesicles from plasma, extracted RNA cargo, 20 sequenced the small RNAs and performed bioinformatic analyses to identify ex-miRNA biomarkers predictive of the stroke subtypes. Sixty-seven miRNAs were significantly variant across the stroke subtypes. A subset of exmiRNAs differed between haemorrhagic and ischaemic strokes, and LASSO analysis could distinguish SAH from the other subtypes with an accuracy of 0.972 ± 0.002. Further analyses predicted 25 miRNA classifiers that stratify IPH from ischaemic stroke with an accuracy of 0.811 ± 0.004 and distinguish haem...Continue Reading

References

Dec 14, 1995·The New England Journal of Medicine·UNKNOWN National Institute of Neurological Disorders and Stroke rt-PA Stroke Study Group
Aug 30, 2008·Lancet Neurology·Mathew J ReevesLynda Lisabeth
Nov 13, 2008·PloS One·Melissa Piper HunterClay B Marsh
Sep 3, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Da-Zhi LiuFrank R Sharp
Nov 21, 2009·Clinical Chemistry·Amy K Saenger, Robert H Christenson
Nov 26, 2009·Biometrical Journal. Biometrische Zeitschrift·Jelle J Goeman
Apr 13, 2010·Brain Research·Enrico De SmaeleAlberto Gulino
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Muller FabbriCarlo M Croce
Nov 23, 2012·Current Drug Targets·Yi-Bing OuyangRona Giffard
Mar 26, 2013·RNA·Kasandra Lovette BurgosKendall Van Keuren-Jensen
Jan 8, 2014·Nature Reviews. Neurology·Michael Tymianski
Jan 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hideyuki ArataMizuo Maeda
Mar 22, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ling Yan LeungColin Alexander Graham
Jun 14, 2014·Carcinogenesis·Olga VaksmanReuven Reich
Jul 25, 2014·Methods in Molecular Biology·Kasandra L Burgos, Kendall Van Keuren-Jensen
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 1, 2015·Genomics, Proteomics & Bioinformatics·Jian ZhangShuangli Mi
Feb 6, 2016·Clinical Chemistry·Tyler F BeckLeslie G Biesecker
Sep 24, 2016·BMC Complementary and Alternative Medicine·Jiangquan LiaoJie Wang
Nov 20, 2016·Neural Regeneration Research·Bridget Martinez, Philip V Peplow
Mar 16, 2017·Stroke; a Journal of Cerebral Circulation·Eric MickJane E Freedman
Mar 23, 2017·Journal of Extracellular Vesicles·Bogdan MateescuEsther N M Nolte-'t Hoen
Jul 19, 2017·Journal of Extracellular Vesicles·Julie A SaugstadFred H Hochberg
Jul 25, 2017·International Journal of Molecular Medicine·Yue-Ting TangQian Wang
Nov 13, 2017·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Brittany DewdneyJonathan Golledge
Nov 14, 2017·The New England Journal of Medicine·Raul G NogueiraUNKNOWN DAWN Trial Investigators
Jan 25, 2018·The New England Journal of Medicine·Gregory W AlbersUNKNOWN DEFUSE 3 Investigators
May 8, 2018·BMC Genomics·Ashish YeriKendall Van Keuren-Jensen
Jul 17, 2018·Nature Biotechnology·Maria D GiraldezMuneesh Tewari
Oct 15, 2018·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Janhavi M ModakLouise D McCullough
Nov 1, 2018·Cell Reports·Paula M GodoyDavid J Erle
Feb 20, 2019·European Journal of Radiology·Adam A DmytriwVitor Mendes Pereira

❮ Previous
Next ❯

Citations

Aug 1, 2020·Biomedicines·Katrine Tang StenzKim Ryun Drasbek
Jan 17, 2021·Molecular Diagnosis & Therapy·Ghada YousifAshfaq Shuaib
Oct 24, 2020·Neurosurgical Review·Ilgiz GareevShiguang Zhao
Dec 29, 2020·Cellular and Molecular Neurobiology·Rais Reskiawan A KadirUlvi Bayraktutan
Feb 20, 2021·Clinical Interventions in Aging·Tao JinYaxing Gui
May 29, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·David de Gonzalo-CalvoUNKNOWN CIBERESUCICOVID Project (COV20/00110, ISCIII)
Aug 8, 2021·International Journal of Molecular Sciences·Anthony FringuelloMichael W Graner

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA Assay
PCR
biopsy

Software Mentioned

exoRNeasy
R
LASSO
STAR
cutadapt
ENSEMBL
bcltofastq
Bowtie2
DESeq
DESeq2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Bengt ZöllerKristina Sundquist
Bangladesh Medical Research Council Bulletin
M A HannanH U Ahmed
Cerebrovascular Diseases
Martin S Dennis
© 2021 Meta ULC. All rights reserved