Extracellular mycobacterial DNA-binding protein 1 participates in mycobacterium-lung epithelial cell interaction through hyaluronic acid.

The Journal of Biological Chemistry
Keiko AokiKazuo Kobayashi

Abstract

Mycobacterium tuberculosis infects not only host macrophages but also nonprofessional phagocytes, such as alveolar epithelial cells. Glycosaminoglycans (GAGs) are considered as the component of mycobacterial adherence to epithelial cells. Here we show that extracellularly occurring mycobacterial DNA-binding protein 1 (MDP1) promotes mycobacterial infection to A549 human lung epithelial cells through hyaluronic acid (HA). Both surface plasmon resonance analysis and enzyme-linked immunosorbent assay revealed that MDP1 bound to HA, heparin, and chondroitin sulfate. Utilizing synthetic peptides, we next defined heparin-binding site of 20 amino acids from 31 to 50 of MDP1, which is responsible for the specific DNA-binding site of MDP1. MDP1 bound to A549 cells, and exogenous DNA and HA interfered with the interaction. The binding was also abolished by treatment of A549 cells with hyaluronidase, suggesting that HA participates in the MDP1-A549 cell interaction. Adherence of bacillus Calmette-Guérin (BCG) and M. tuberculosis to A549 cells was inhibited by addition of HA, DNA, and anti-MDP1 antibody, showing that MDP1 participates in the interaction between mycobacteria-alveolar epithelial cells. Simultaneous treatment of intratracheal...Continue Reading

References

Jun 6, 1991·Nature·C K StoverG F Hatfull
Apr 1, 1988·Trends in Biochemical Sciences·M B Schmid
Sep 1, 1987·Microbiological Reviews·K Drlica, J Rouviere-Yaniv
Sep 1, 1987·Applied Biochemistry and Biotechnology·V C YangR Langer
Mar 18, 1970·Biochimica Et Biophysica Acta·T Ohya, Y Kaneko
Apr 1, 1996·Infection and Immunity·L E Bermudez, J Goodman
Sep 1, 1996·The Journal of Experimental Medicine·F D MenozziC Locht
Jul 1, 1997·The Journal of Clinical Investigation·M F Kagnoff, L Eckmann
Feb 28, 1998·The Journal of Biological Chemistry·M NaitoT Yamada
Aug 26, 1998·Scandinavian Journal of Immunology·M NaitoT Yamada
Jan 23, 1999·Molecular & General Genetics : MGG·B H LeeT Dick
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y ShimojiA Rambukkana
Dec 22, 1999·Journal of Bacteriology·G Delogu, M J Brennan
Aug 22, 2000·The Journal of Experimental Medicine·U AnderssonK J Tracey
Mar 13, 2001·Annual Review of Immunology·J L Flynn, J Chan
Jul 21, 2001·The New England Journal of Medicine·P M Small, P I Fujiwara
Nov 22, 2001·The Journal of Biological Chemistry·Eva A TurleyLilly Y W Bourguignon
May 10, 2002·The New England Journal of Medicine·Barry R Bloom
Jul 12, 2002·Nature·Paola ScaffidiMarco E Bianchi
Mar 5, 2003·Trends in Immunology·Christian TermeerJan C Simon
Mar 12, 2003·The Journal of Experimental Medicine·Loic CoutteFrancoise Jacob-Dubuisson
Jun 11, 2003·The Journal of Cell Biology·Joanna Cichy, Ellen Puré
Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nobuyuki ShimonoLee W Riley

❮ Previous
Next ❯

Citations

Jun 1, 2012·The Journal of Biological Chemistry·Makoto NikiSohkichi Matsumoto
Sep 18, 2007·Journal of Bacteriology·Tomoya KatsubeKazuo Kobayashi
May 17, 2008·BMC Microbiology·Michelle I PortugalMaria C V Pessolani
Oct 31, 2009·PLoS Pathogens·Yukio HirayamaSohkichi Matsumoto
May 10, 2012·Clinical & Developmental Immunology·Yong LiXiaoming Liu
Oct 16, 2014·Pediatric Rheumatology Online Journal·Kozo Yasui
May 28, 2010·Nihon saikingaku zasshi. Japanese journal of bacteriology·Toshi Nagata, Yukio Koide
Jan 5, 2012·Nihon saikingaku zasshi. Japanese journal of bacteriology·Sohkichi Matsumoto
Oct 31, 2007·Journal of Microbiological Methods·Jonghoon KangDavid G Gorenstein
Jun 11, 2011·Cellular Microbiology·Cristiane de Souza CarvalhoMaximiliano Gabriel Gutierrez
Jul 8, 2009·Biochemical and Biophysical Research Communications·Shintaro SetoYukio Koide
May 28, 2005·Microbes and Infection·Cristiana Soares de LimaMaria Cristina Vidal Pessolani
Mar 4, 2008·Microbiology·D PecharkiA Aa Scheie
Jun 21, 2011·Biochemical and Biophysical Research Communications·Keiko SugayaYukio Koide
May 26, 2010·Genetic Vaccines and Therapy·Babak JalilianSohkichi Matsumoto
Feb 21, 2006·FEMS Immunology and Medical Microbiology·Sheyla M R C BonfimMaristela Pereira
Jul 20, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N SritharanM Sritharan
Oct 8, 2016·Trends in Microbiology·Jesús Arenas, Jan Tommassen
Jan 11, 2013·Memórias do Instituto Oswaldo Cruz·André Alves DiasMaria Cristina Vidal Pessolani
Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
May 24, 2013·Japanese Journal of Infectious Diseases·Yoshiro YamashitaYasuko Tsunetsugu-Yokota
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kasper M A RouschopSandrine Florquin
Jan 19, 2008·The Journal of Biological Chemistry·Tiina A JokelaRaija H Tammi
Aug 6, 2014·Molecular and Cellular Biochemistry·Pooja VirIndu Verma
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sohkichi MatsumotoKazuo Kobayashi
Apr 22, 2005·The Journal of Biological Chemistry·Alka RaoAnand Ranganathan

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.