Extracellular neutral protease from Arthrospira platensis: Production, optimization and partial characterization.

International Journal of Biological Macromolecules
Jihen ElleuchSlim Abdelkafi

Abstract

Proteases are industrially important catalysts. They belong to a complex family of enzymes that perform highly focused proteolysis functions. Given their potential use, there has been renewed interest in the discovery of proteases with novel properties and a constant thrust to optimize the enzyme production. In the present study, a novel extracellular neutral protease produced from Arthrospira platensis was detected and characterized. Its proteolytic activity was strongly activated by β-mercaptoethanol, 5,5-dithio-bis-(2-nitrobenzoic acid) and highly inhibited by Hg2+ and Zn2+ metal ions which support the fact that the studied protease belongs to the cysteine protease family. Using statistical modelling methodology, the logistic model has been selected to predict A. platensis growth-kinetic values. The optimal culture conditions for neutral protease production were found using Box-Behnken Design. The maximum experimental protease activities (159.79 U/mL) was achieved after 13 days of culture in an optimized Zarrouk medium containing 0.625 g/L NaCl, 0.625 g/L K2HPO4 and set on 9.5 initial pH. The extracellular protease of A. platensis can easily be used in the food industry for its important activity at neutral pH and its low pr...Continue Reading

References

Nov 1, 1994·International Journal of Food Microbiology·J Baranyi, T A Roberts
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M B RaoV V Deshpande
Aug 24, 2004·Extremophiles : Life Under Extreme Conditions·Xiao-Ge ChenStephen Tiong-Lee Tay
Mar 31, 2010·Applied Biochemistry and Biotechnology·Daniela de Araújo VianaAna Lúcia Figueiredo Porto
Aug 10, 2010·Transplantation Proceedings·A G BreiteR C McCarthy
Oct 13, 2010·Journal of Bioscience and Bioengineering·Shakilanishi SundararajanShanthi Chittibabu
Mar 1, 2011·Phytochemistry·Malik Muhammad HashimChen Xiaohong
Apr 7, 2012·Journal of Hazardous Materials·Mayla Santos RodriguesAttilio Converti
Jul 27, 2015·International Journal of Biological Macromolecules·Paula Monteiro SouzaPatrizia Perego
Apr 18, 2016·International Journal of Biological Macromolecules·Ahmad HomaeiReyhaneh Sariri
May 18, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Thiago Pajeú NascimentoAttilio Converti
Jul 28, 2016·Toxicon : Official Journal of the International Society on Toxinology·Jihen ElleuchRaida Z Zghal
Apr 1, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Paula Monteiro SouzaAdalberto Pessoa Junior
May 14, 2017·Bioprocess and Biosystems Engineering·Thiago Pajeú NascimentoAttilio Converti
Jul 29, 2017·International Journal of Biological Macromolecules·Faten Ben AmorImen Fendri
Feb 2, 2018·Food Research International·Jessika Gonçalves Dos Santos Aguilar, Hélia Harumi Sato
Sep 22, 2018·Bioinformation·Manju MohanKothanam Kuzhiyil Elyas
Jun 1, 2015·Journal, Genetic Engineering & Biotechnology·Aysha KamranMustafa Kamal
Jan 22, 2019·International Journal of Biological Macromolecules·Poorani GurumalleshShanmugaprakash Muthusamy
Feb 9, 2019·Journal, Genetic Engineering & Biotechnology·B K M LakshmiK P J Hemalatha
Jul 3, 2019·Frontiers in Bioengineering and Biotechnology·Abdul RazzaqMuhammad Ashraf
Jun 21, 2020·Environmental Science and Pollution Research International·Vinod Kumar Nathan, Mary Esther Rani

❮ Previous
Next ❯

Citations

Aug 15, 2021·The Science of the Total Environment·Jihen ElleuchSlim Abdelkafi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved