Extracellular nucleic acids in cultures of long-term cultivated eukaryotic cells

Annals of the New York Academy of Sciences
E S MorozkinValentin V Vlassov

Abstract

Investigation of the kinetics of nucleic acid release by HeLa (human cervical carcinoma cell line) and A431 (human squamous carcinoma cell line) cells is presented. The released DNA and RNA were shown to accumulate in culture medium and at the cell surface. A portion of cell surface bound RNA can be eluted with PBS/EDTA. Mild trypsin treatment is required for complete detachment of cell surface bound RNA and cell surface bound DNA. Electrophoretic analysis reveals characteristic patterns of cell-associated and free RNA and DNA molecules.

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Citations

Dec 29, 2012·PLoS Genetics·Kuntal Mukherjee, Francesca Storici
Apr 17, 2012·Expert Opinion on Biological Therapy·Elena Y RykovaPavel P Laktionov
Apr 17, 2012·Expert Opinion on Biological Therapy·Anna V CherepanovaPavel P Laktionov
Jul 4, 2012·Immunobiology·Anna V CherepanovaPavel P Laktionov
Nov 17, 2006·Annals of the New York Academy of Sciences·Evgeniy S MorozkinPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Tatyana E SkvortsovaPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Evgeniy S MorozkinPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Vera S Mal'shakovaPavel P Laktionov
Sep 10, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O E Bryzgunova, P P Laktionov
Jan 13, 2011·FEMS Microbiology Reviews·Elizabeth Skippington, Mark A Ragan
Jul 10, 2016·Cancer Metastasis Reviews·A R ThierryM Stroun
Jun 15, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Valentin V VlassovElena Y Rykova
Jan 14, 2020·British Journal of Cancer·Amaelle OtandaultAlain R Thierry

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