Extracellular Pectinase from a Novel Bacterium Chryseobacterium indologenes Strain SD and Its Application in Fruit Juice Clarification

Enzyme Research
Karabi RoyMd Towhid Hossain

Abstract

Pectinase is one of the important enzymes of industrial sectors. Presently, most of the pectinases are of plant origin but there are only a few reports on bacterial pectinases. The aim of the present study was to isolate a novel and potential pectinase producing bacterium as well as optimization of its various parameters for maximum enzyme production. A total of forty bacterial isolates were isolated from vegetable dump waste soil using standard plate count methods. Primary screening was done by hydrolysis of pectin. Pectinase activity was determined by measuring the increase in reducing sugar formed by the enzymatic hydrolysis of pectin. Among the bacterial isolates, the isolate K6 exhibited higher pectinase activity in broth medium and was selected for further studies. The selected bacterial isolate K6 was identified as Chryseobacterium indologenes strain SD. The isolate was found to produce maximum pectinase at 37°C with pH 7.5 upon incubation for 72 hours, while cultured in production medium containing citrus pectin and yeast extract as C and N sources, respectively. During enzyme-substrate reaction phase, the enzyme exhibited its best activity at pH of 8.0 and temperature of 40°C using citrus pectin as substrate. The pecti...Continue Reading

References

Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
May 20, 2017·International Scholarly Research Notices·Muhammad Hariadi NawawiWan Zuhainis Saad

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Citations

Nov 6, 2018·Folia Microbiologica·Bhagwan Rekadwad, Juan M Gonzalez
Apr 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Octavio Roldán-PadrónTeresa García-Gasca
Dec 1, 2020·Critical Reviews in Food Science and Nutrition·Joanne Yi Hui ToyShao-Quan Liu

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Datasets Mentioned

BETA
KY684254

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
Molecular Evolutionary Genetics Analysis
BioEdit
Basic Local Alignment Search Tool ( BLAST )

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