PMID: 9438999Jan 24, 1998Paper

Extracellular production of an intact and biologically active human growth hormone by the Bacillus brevis system

Journal of Industrial Microbiology & Biotechnology
T KajinoS Udata

Abstract

The characteristic features of the Bacillus brevis system are very high productivity of heterologous proteins and very low extracellular protease activity. However, degradation of some heterologous proteins, especially mammalian proteins, can be observed and resulted in a lowering of protein productivity. By using a mutant expressing low levels of proteases and the addition of EDTA to the medium, intact human growth hormone (hGH) was successfully produced with the B. brevis system. Signal peptide modification with higher basicity in the amino terminal region and higher hydrophobicity in the middle region brought about a twelve-fold increase in hGH production. The hGH yield was further elevated to 240 mg L-1 by optimization of culture conditions. Thus, biologically active and mature hGH can be efficiently produced directly in the medium with the B. brevis system.

Citations

Nov 26, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·T TakiiK Onozaki
Jan 5, 2000·Applied and Environmental Microbiology·T KajinoY Yamada
Jan 28, 2004·Bioscience, Biotechnology, and Biochemistry·Yasuhiro Kashima, Shigezo Udaka
Mar 17, 2010·Journal of Bioscience and Bioengineering·Abelardo MargollesPatricia Ruas-Madiedo
Feb 19, 2009·Biotechnology Progress·Tunçer H OzdamarPinar Calik
Jun 25, 2015·Bioprocess and Biosystems Engineering·Burcu ŞahinTunçer H Özdamar
Mar 31, 2004·Canadian Journal of Microbiology·Marcus SchallmeyOwen P Ward

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