PMID: 9528662Apr 7, 1998Paper

Extracellular ribonuclease from Rhizopus stolonifer: characteristics of an atypical--guanylic acid preferential--enzyme from ribonuclease T2 family

Biochimica Et Biophysica Acta
R Chacko, V Shankar

Abstract

An extracellular ribonuclease from Rhizopus stolonifer (designated as RNase Rs) was purified to homogeneity by chromatography on DEAE-cellulose followed by CM-cellulose. The Mr of the purified enzyme determined by gel filtration and SDS-PAGE is 25,000 and 28,200, respectively. RNase Rs is a glycoprotein and contains 10.5% neutral sugar. It is an acidic protein with a pI of 5.0 and has a blocked N-terminus. The optimum pH and temperature are 5.5 and 45 degrees C, respectively. RNase Rs shows high stability between pH 6.0-10.0. Divalent cations like Zn2+, Hg2+ and Cu2+ inhibit the enzyme activity whereas, mononucleotides does not have any significant effect. The enzyme cleaves RNA to 3'-mononucleotides via 2',3'-cyclic nucleotides, with preferential liberation of 2',3'-cyclic GMP, suggesting that RNase Rs is a guanylic acid preferential cyclizing RNase. Moreover, cyclic nucleotides generated are highly resistant to further hydrolysis.

References

Sep 1, 1979·Chemical & Pharmaceutical Bulletin·A SandaM Irie
Jan 1, 1990·Methods in Enzymology·D E Garfin
Jan 1, 1990·Methods in Enzymology·C M Stoscheck
Oct 1, 1988·European Journal of Biochemistry·Y KawataH Tamaoki
Apr 1, 1969·Archives of Biochemistry and Biophysics·M TomoyedaT Yoshino
Nov 1, 1971·Journal of Biochemistry·T Komiyama, M Irie
Nov 1, 1967·Journal of Biochemistry·M Irie
Jun 1, 1969·Journal of Biochemistry·M Irie
Jun 1, 1980·Canadian Journal of Biochemistry·H HoritsuM Tomoyeda
Jan 1, 1993·Critical Reviews in Biotechnology·L G Reddy, V Shankar
Jan 1, 1972·Methods in Enzymology·W R Gray
Jan 1, 1972·Methods in Enzymology·A F Habeeb
Mar 1, 1993·World Journal of Microbiology & Biotechnology·P S ApteV Shankar

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Citations

Jul 22, 2010·The Journal of General and Applied Microbiology·Xiangli WuTzi Bun Ng
Oct 5, 2015·World Journal of Microbiology & Biotechnology·E Esin Hameş, Tuğçe Demir
May 9, 2001·Biochimica Et Biophysica Acta·R A DeshpandeV Shankar
May 22, 2003·Biochimica Et Biophysica Acta·Rajashree A DeshpandeVepatu Shankar

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