PMID: 11319031Apr 25, 2001Paper

Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate

The Plant Journal : for Cell and Molecular Biology
A E RichardsonJ E Hayes

Abstract

Phosphorus (P) deficiency in soil is a major constraint for agricultural production worldwide. Despite this, most soils contain significant amounts of total soil P that occurs in inorganic and organic fractions and accumulates with phosphorus fertilization. A major component of soil organic phosphorus occurs as phytate. We show that when grown in agar under sterile conditions, Arabidopsis thaliana plants are able to obtain phosphorus from a range of organic phosphorus substrates that would be expected to occur in soil, but have only limited ability to obtain phosphorus directly from phytate. In wild-type plants, phytase constituted less than 0.8% of the total acid phosphomonoesterase activity of root extracts and was not detectable as an extracellular enzyme. By comparison, the growth and phosphorus nutrition of Arabidopsis plants supplied with phytate was improved significantly when the phytase gene (phyA) from Aspergillus niger was introduced. The Aspergillus phytase was only effective when secreted as an extracellular enzyme by inclusion of the signal peptide sequence from the carrot extensin (ex) gene. A 20-fold increase in total root phytase activity in transgenic lines expressing ex::phyA resulted in improved phosphorus n...Continue Reading

References

Jan 1, 1996·Advances in Applied Microbiology·R J Wodzinski, A H Ullah
Mar 1, 1997·The Biochemical Journal·S MaugenestA M Lescure
Mar 10, 1998·Plant Physiology·D P SchachtmanS M Ayling
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·K. G. Raghothama
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D ValvekensM Van Lijsebettens

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Citations

Apr 1, 2009·Applied Biochemistry and Biotechnology·Bijender Singh, T Satyanarayana
Nov 25, 2003·Biochemical and Biophysical Research Communications·Edward J Mullaney, Abul H J Ullah
Mar 12, 2013·Journal of Biotechnology·Rumei ChenYunliu Fan
Mar 22, 2002·Trends in Plant Science·Henrik Brinch-PedersenPreben Bach Holm
Feb 22, 2002·Current Opinion in Plant Biology·Natasha Grotz, Mary Lou Guerinot
Jan 14, 2004·Journal of Experimental Botany·José Manuel Franco-ZorrillaJavier Paz-Ares
Aug 16, 2011·Journal of Experimental Botany·Peter J Gregory, Timothy S George
May 25, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Benjamin L TurnerIan D McKelvie
May 4, 2004·Plant Physiology·Jennifer L TomschaMark J Guiltinan
Apr 25, 2006·Plant Physiology·Georgia DrakakakiEva Stoger
Feb 27, 2007·Plant Physiology·Ballachanda N DevaiahKashchandra G Raghothama
Jul 10, 2009·Plant Physiology·Xiurong WangHong Liao
Jan 5, 2006·Applied and Environmental Microbiology·Keri WangGeorge Lazarovits
Oct 3, 2012·Eukaryotic Cell·Giek Far ChanNoor Aini Abdul Rashid
Jan 1, 2002·The Arabidopsis Book·Yves Poirier, Marcel Bucher
Mar 4, 2014·Annual Review of Plant Biology·Damar Lizbeth López-ArredondoLuis Herrera-Estrella
Aug 20, 2015·International Journal of Molecular Sciences·Bargaz AdnaneCarlsson Georg
Apr 4, 2015·The Journal of General and Applied Microbiology·Eduardo Rezende GraminhoTakayuki Hoshino
Mar 29, 2014·International Journal of Microbiology·Prashant SinghSanjeev Agrawal
Nov 2, 2014·Frontiers in Plant Science·Adeline BecquerPlassard Claude
Sep 3, 2005·Protein Expression and Purification·Wing-Lee ChanBoon L Lim
Oct 27, 2004·Trends in Plant Science·Carla A Ticconi, Steffen Abel
Jan 1, 2013·Annual Review of Animal Biosciences·Xin Gen LeiMichael J Azain
Nov 13, 2007·Biochemical and Biophysical Research Communications·Xiaoxue WangYunliu Fan
Dec 15, 2010·Annals of Botany·Stephen L ByrneSusanne Barth
Mar 17, 2010·Annals of Botany·Xiurong WangHong Liao

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