PMID: 9171378Jun 1, 1997Paper

Extracellular secretion of Escherichia coli heat-stable enterotoxin I across the outer membrane

Journal of Bacteriology
H YamanakaK Okamoto

Abstract

Escherichia coli heat-stable enterotoxin Ip (STIp) is an extracellular toxin consisting of 18 amino acid residues that is synthesized as a precursor of pre (amino acid residues 1 to 19), pro (amino acid residues 20 to 54), and mature (amino acid residues 55 to 72) regions. The precursor synthesized in the cytoplasm is translocated across the inner membrane by the general export pathway consisting of Sec proteins. The pre region functions as a leader peptide and is cleaved during translocation. However, it remains unknown how the resulting peptide (pro-mature peptide) translocates across the outer membrane. In this study, we investigated the structure of the STIp that passes through the outer membrane to determine how it translocates through the outer membrane. The results showed that the pro region is cleaved in the periplasmic space. The generated peptide becomes the mature form of STIp, which happens to have disulfide bonds, which then passes through the outer membrane. We also showed that STIp with a carboxy-terminal peptide consisting of 3 amino acid residues passes through the outer membrane, whereas STIp with a peptide composed of 37 residues does not. Amino acid analysis of mutant STIp purified from culture supernatant r...Continue Reading

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Citations

Apr 1, 2000·The Journal of Biological Chemistry·I Batisson, M der Vartanian
Oct 27, 2004·Microbiology and Immunology·Hiroyasu YamanakaKeinosuke Okamoto
Aug 30, 2014·FEMS Microbiology Reviews·Joseph J GillespieAbdu F Azad
Jul 10, 2012·Journal of Bacteriology·Simran J KaurAbdu F Azad
Aug 24, 2013·Journal of Biomolecular Structure & Dynamics·Philipp R WeiglmeierPaul Rösch
Nov 8, 2001·Journal of Bacteriology·H YamanakaK Okamoto
Sep 23, 2008·Journal of Bacteriology·Hiroyasu YamanakaKeinosuke Okamoto
Mar 17, 2010·Infection and Immunity·Arne TaxtPål Puntervoll
Oct 14, 2016·EcoSal Plus·J Daniel DubreuilDieter M Schifferli

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