PMID: 9532741Apr 9, 1998Paper

Extracellular superoxide dismutase from Streptococcus pyogenes type 12 strain is manganese-dependent

FEMS Microbiology Letters
D GerlachS Vettermann

Abstract

Highly purified extracellular superoxide dismutase was obtained from Streptococcus pyogenes strain 12,714 (type 12) by adsorption of culture supernatant on phenyl-Sepharose following preparative isoelectric focusing of eluates and a final gel filtration purification on Superdex 200. The purified superoxide dismutase of S. pyogenes was found to be a homodimer. The monomeric protein had a molecular mass of 22,442 Da and an isoelectric point of 4.0. The enzymatic activity was strongly manganese-dependent. The N-terminal sequence of the purified mature protein was AIILPELPYAYDALEPQUFDA and corresponded to the first amino acids following the methionine initiation codon with no evidence of a leader sequence for the mature protein. The DNA sequence of the superoxide dismutase gene of strain 12,714 was found to be almost identical to the corresponding sequences reported in the gene bank data from other S. pyogenes serotypes and showed strong homology to superoxide dismutases from other Gram-positive bacteria.

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Citations

Jul 30, 2003·Free Radical Biology & Medicine·Cheryl L FattmanTim D Oury
Nov 15, 2007·Antioxidants & Redox Signaling·Fei GaoTim D Oury
Sep 29, 2011·Journal of Bacteriology·Renata GrifantiniIoannis Gryllos
May 23, 2009·Biological Trace Element Research·Praveen RishiRam Prakash Tiwari
Nov 7, 2000·Biotechnology and Bioengineering·M KreinerL M Harvey
Mar 19, 2009·Archives of Environmental Contamination and Toxicology·Tiejuan ShaoHang Min
Oct 9, 2002·Microbiology·Nicholas S JakubovicsHoward F Jenkinson
Jun 10, 2004·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Zhenmei LüYing Xia
Mar 19, 2015·Life·Paulo OliveiraPaula Tamagnini
Jun 26, 2012·FEMS Microbiology Letters·Ying ZhangYang Wang
Feb 12, 2015·FEMS Microbiology Reviews·Anna HenninghamJason N Cole
Mar 25, 2004·FEMS Immunology and Medical Microbiology·David J McMillanKadaba S Sriprakash
Mar 2, 2019·The Biochemical Journal·Nicholas S Jakubovics
May 10, 2019·Frontiers in Cellular and Infection Microbiology·Maribasappa KarchedSirkka Asikainen
Jun 28, 2003·FEMS Microbiology Reviews·David G Kehres, Michael E Maguire
Mar 9, 2018·ACS Infectious Diseases·Sabrina S Schatzman, Valeria C Culotta

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