Extracellular vesicle microRNA cargo is correlated with HPV status in oropharyngeal carcinoma

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Ben PeacockStuart Hunt

Abstract

The incidence of human papilloma virus positive (HPV+ ) oropharyngeal squamous cell carcinoma (OPSCC) has increased rapidly in recent decades. These tumours have a favourable outcome compared to HPV-negative (HPV- ) OPSCC. However, HPV+ tumours are more likely to metastasise to distant sites, suggesting a difference in how these tumour subtypes interact with the metastatic niche. Extracellular vesicles (EVs) have emerged as important players in cell-to-cell communication and are a potential source of biomarkers for cancer diagnosis. This study aims to characterise the microRNA cargo of small EVs released by HPV+ and HPV- OPSCC cell lines. Extracellular vesicles produced by HPV+ (SCC2 and SCC90) and HPV- (SCC72 an SCC89) OPSCC cells were characterised by tunable resistive pulse sensing (TRPS) and western blotting. RNA was extracted from EVs and analysed by small RNA sequencing. A bioinformatics approach was used to identify EV miRNA signatures associated with HPV status. HPV- OPSCC cells produced more EVs than HPV+ OPSCC cells. EVs were positive for the common EV markers CD63, CD9 and TSG101. Unbiased hierarchical clustering analysis of EV miRNA cargo revealed that samples clustered based on HPV status. 14 miRNA were enriched in...Continue Reading

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Citations

Jun 30, 2019·Cancers·Giulia ChiabottoGiovanni Camussi
Jun 21, 2019·Cells·Fabio Seiti Yamada YoshikawaLuanda Mara da Silva Oliveira
Oct 2, 2019·International Journal of Molecular Sciences·Zuzanna NowickaWojciech Fendler
Jan 19, 2021·Seminars in Cancer Biology·Leanne L LeungKatie Meehan
Nov 11, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lihong HeJuan Xia
Nov 8, 2020·International Journal of Molecular Sciences·Xinyu QuKatie Meehan
Feb 10, 2021·Journal of Personalized Medicine·Maria MeniniPaolo Pesce
Jul 1, 2021·Maxillofacial Plastic and Reconstructive Surgery·Cosmin Ioan FaurMihaela Hedesiu

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