Extracellular vesicles concentration is a promising and important parameter for industrial bioprocess monitoring

Biotechnology Journal
Apolonija Bedina ZavecGregor Anderluh

Abstract

Extracellular vesicles (EVs) are membrane vesicles that are produced by cells to be released into their microenvironment. In this study, we present the EV concentration as a new factor for optimization of industrial bioprocess control. The release of EVs depends on many cell properties, including cell activation and stress status, and cell death. Therefore, the EV concentration might provide a readout for identification of the cell state and the conditions during a bioprocess. Our data show that the EV concentration increased during the bioprocess, which indicated deteriorating conditions in the bioreactor. This increase in EV concentration in the fermentation broth was the consequence of two different processes: cell activation, and cell death. However, the release of EVs from activated living cells had a much weaker impact on EV concentration in the bioreactor than those released during cell death. EVs and cells in the bioprocess environment were quantified by flow cytometry. The most accurate data were obtained directly from unprocessed samples, making the monitoring of the EV concentration a rapid, easy, and cheap method. These EV concentrations reflect the conditions in the bioreactor and provide new information regarding ...Continue Reading

References

Jan 16, 2009·Trends in Cell Biology·Emanuele CocucciJacopo Meldolesi
Jun 6, 2009·Nature Reviews. Immunology·Clotilde ThéryElodie Segura
Oct 26, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Esther N M Nolte-'t HoenMarca H M Wauben
Dec 1, 2011·International Journal of Nanomedicine·Vid SuštarVeronika Kralj-Iglič
Nov 6, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michael C LarsonCheryl A Hillery
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Apr 16, 2013·Nature Reviews. Drug Discovery·Samir EL AndaloussiMatthew J A Wood
Aug 22, 2013·Microscopy Research and Technique·Timothy MooreVitaly Vodyanoy
Sep 7, 2013·Journal of Extracellular Vesicles·Stephen J Gould, Graça Raposo
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Marina ColomboClotilde Théry
Feb 17, 2015·Trends in Cell Biology·Emanuele Cocucci, Jacopo Meldolesi
Jul 5, 2015·Journal of Extracellular Vesicles·Carsten FrühbeisEva-Maria Krämer-Albers

❮ Previous
Next ❯

Citations

Jun 12, 2016·Journal of Chromatography. a·Petra SteppertAlois Jungbauer
Feb 8, 2020·Biotechnology Journal·Guiying LiFang Yang
Mar 31, 2021·Free Radical Biology & Medicine·Cristina Mas-Bargues, Consuelo Borrás
May 4, 2021·Applied Microbiology and Biotechnology·Christoph KeysbergThomas Noll

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.