Extracellular vesicles from deciduous pulp stem cells recover bone loss by regulating telomerase activity in an osteoporosis mouse model.

Stem Cell Research & Therapy
Soichiro SonodaTakayoshi Yamaza

Abstract

Systemic transplantation of stem cells from human exfoliated deciduous teeth (SHED) recovers bone loss in animal models of osteoporosis; however, the mechanisms underlying this remain unclear. Here, we hypothesized that trophic factors within SHED-releasing extracellular vesicles (SHED-EVs) rescue osteoporotic phenotype. EVs were isolated from culture supernatant of SHED. SHED-EVs were treated with or without ribonuclease and systemically administrated into ovariectomized mice, followed by the function of recipient bone marrow mesenchymal stem cells (BMMSCs) including telomerase activity, osteoblast differentiation, and sepmaphorine-3A (SEMA3A) secretion. Subsequently, human BMMSCs were stimulated by SHED-EVs with or without ribonuclease treatment, and then human BMMSCs were examined regarding the function of telomerase activity, osteoblast differentiation, and SEMA3A secretion. Furthermore, SHED-EV-treated human BMMSCs were subcutaneously transplanted into the dorsal skin of immunocompromised mice with hydroxyapatite tricalcium phosphate (HA/TCP) careers and analyzed the de novo bone-forming ability. We revealed that systemic SHED-EV-infusion recovered bone volume in ovariectomized mice and improved the function of recipient B...Continue Reading

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Citations

Jan 14, 2021·Stem Cell Research & Therapy·Ratih YuniarthaTomoaki Taguchi
Jun 4, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Soichiro SonodaTakayoshi Yamaza

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Methods Mentioned

BETA
flow cytometry
protein assay
enzyme-linked immunosorbent assay
ELISA
histone acetylation

Software Mentioned

ImageJ
CTAn
GraphPad
PRISM

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