Extracellular vesicles in diabetes mellitus induce alterations in endothelial cell morphology and migration

Journal of Translational Medicine
Sharon F WuMichele K Evans

Abstract

Inflammation-related atherosclerotic peripheral vascular disease is a major end organ complication of diabetes mellitus that results in devastating morbidity and mortality. Extracellular vesicles (EVs) are nano-sized particles that contain molecular cargo and circulate in the blood. Here, we examined EV protein cargo from diabetic individuals and whether these EVs cause functional changes in endothelial cells. We quantified inflammatory protein levels in plasma-derived EVs from a longitudinal cohort of euglycemic and diabetic individuals and used in vitro endothelial cell biological assays to assess the functional effects of these EVs with samples from a cross-sectional cohort. We found several significant associations between EV inflammatory protein levels and diabetes status. The angiogenic factor, vascular endothelial growth factor A (VEGF-A), was associated with diabetes status in our longitudinal cohort. Those with diabetes mellitus had higher EV VEGF-A levels compared to euglycemic individuals. Additionally, EV levels of VEGF-A were significantly associated with homeostatic model assessment of insulin resistance (HOMA-IR) and β-cell function (HOMA-B). To test whether EVs with different inflammatory cargo can demonstrate d...Continue Reading

References

Apr 1, 1987·Diabetes/metabolism Reviews·K PyöräläM Uusitupa
Dec 15, 1994·Annals of Internal Medicine·T V PernegerM J Klag
Feb 1, 1997·Nature Medicine·J A Ware, M Simons
Dec 1, 1998·Neuroepidemiology·T G LukovitsT M Gorelick
May 10, 2001·The Journal of Clinical Endocrinology and Metabolism·C WeyerP A Tataranni
Jun 28, 2002·Cancer Cell·Kari Alitalo, Peter Carmeliet
Nov 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Harold F Dvorak
May 27, 2004·Diabetes Care·Tara M WallaceDavid R Matthews
Apr 20, 2005·International Journal of Cardiology·Dimitris TousoulisChristodoulos Stefanadis
Aug 23, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Christian P FischerBente K Pedersen
Mar 15, 2006·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Angelo AvogaroSaula de Kreutzenberg
Apr 14, 2006·Neoplasia : an International Journal for Oncology Research·Giulia TarabolettiVincenza Dolo
Mar 31, 2007·Circulation Research·Laurent LamaliceJacques Huot
Jun 3, 2008·Nature Cell Biology·Petra BeliMetello Innocenti
Oct 22, 2008·Progress in Retinal and Eye Research·Barbara WirostkoRafael Simó
Nov 4, 2008·Trends in Molecular Medicine·Muhammad RizviSubrata Chakrabarti
Jan 18, 2012·Current Opinion in Endocrinology, Diabetes, and Obesity·Ming-Lin Liu, Kevin Jon Williams
Mar 15, 2013·The Journal of Clinical Investigation·James K Liao
Apr 16, 2013·Nature Reviews. Drug Discovery·Samir EL AndaloussiMatthew J A Wood
Jun 15, 2013·The Journal of Biological Chemistry·Jing LiChen-Yu Zhang
Sep 7, 2013·Journal of Extracellular Vesicles·Jason Webber, Aled Clayton
Jun 7, 2014·Journal of Zhejiang University. Science. B·Barbara Ruszkowska-CiastekDanuta Rość
Jul 12, 2014·Tissue Engineering. Part B, Reviews·Lucas T VuPadmavathy Rajagopalan
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever
Jun 13, 2015·Nutrition & Metabolism·Yaqin ZhangDalong Zhu
Nov 11, 2015·Nature Reviews. Nephrology·Merlin C ThomasPaul Zimmet
Jan 30, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Devi Prasadh RamakrishnanRoy L Silverstein
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joanna KowalClotilde Théry
Nov 11, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Sheyu LiJianwei Li
Jan 4, 2017·Journal of Diabetes·Yi Shi, Paul M Vanhoutte
Apr 7, 2017·BMC Medicine·Samantha Perakis, Michael R Speicher
Aug 2, 2017·Neural Regeneration Research·Xian-Bin KongZhong-Lei Sun
Aug 3, 2017·Molecular & Cellular Proteomics : MCP·Pia LarssenMasood Kamali-Moghaddam
Aug 18, 2017·Journal of Extracellular Vesicles·Lucas TrepsJulie Gavard

❮ Previous
Next ❯

Citations

Aug 30, 2020·Cells·Sherin SaheeraPrasanna Krishnamurthy
Feb 9, 2021·Frontiers in Endocrinology·Jie LiuYi Yang
May 1, 2021·International Journal of Molecular Sciences·Allison MathiesenAnca Dobrian
Jun 3, 2021·Diagnostics·Robert J Asaro, Pedro Cabrales
Oct 6, 2021·Journal of Extracellular Vesicles·Yujia YinXipeng Wang

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
electron microscopy
Assay
Fluorescence

Software Mentioned

NanoSight
R
ExoQuick
NTA

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Sub-cellular Biochemistry
Pamali FonsekaSuresh Mathivanan
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Petra HirsovaHarmeet Malhi
International Journal of Molecular Sciences
Tünde SzatmáriKatalin Lumniczky
Antioxidants & Redox Signaling
Marine MallociM Carmen Martínez
© 2022 Meta ULC. All rights reserved