Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes

The Journal of Venomous Animals and Toxins Including Tropical Diseases
Leticia Gomes de PontesLucilene Delazari Dos Santos

Abstract

Extracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (Bubalus bubalis) sera infected with Theileria spp. Infected animals were identified through optical microscopy and PCR. EVs were isolated from buffalo sera by size-exclusion chromatography and characterized using western blotting analysis, nanoparticle tracking analysis and transmission electron microscopy. Subsequently, the proteins from isolated vesicles were characterized by mass spectrometry. EVs from buffalo sera have shown sizes in the 124-140 nm range and 306 proteins were characterized. The protein-protein interaction analysis has evidenced biological processes and molecular function associated with signal transduction, binding, regulation of metabolic processes, transport, catalytic activity and response to acute stress. Five proteins have been shown to be differentially expressed between the control group and that infected with Theileria spp., all acting in the oxidative stress pathway. EVs from buffaloes infected with Theileria spp. were successfully isolated and characterized. T...Continue Reading

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Methods Mentioned

BETA
PCR
electrophoresis
size-exclusion chromatography
fluorescence imager
transmission electron microscopy
PCA
size exclusion chromatography
transmission

Software Mentioned

ImageJ
PANTHER
MetaboAnalyst
STRING
Proteome Discoverer

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