Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study

Biology of Sex Differences
Muthuvel JayachandranJohn C Lieske

Abstract

The lifetime incidence of kidney stones is about two times greater in men compared to women. Extracellular vesicles (EVs) shed from activated cells are present in the urine and may reflect or even mediate renal physiology and/or pathology. This study was designed to standardize methodology to characterize urinary EVs by digital flow cytometry and to identify possible sex differences in EVs in persons with and without their first symptomatic kidney stones. Twenty-four-hour urine collections were obtained from persons presenting with their first kidney stone episode (n = 50 women, 60 men; age 19-76 years) and sex- and age-matched controls from the general population (n = 24 women, 36 men). Standardization: Size of EV was variable within all groups. EV positivity was verified with two fluorophores for surface phosphatidylserine and/or using two different protein markers specific for renal-specific cells. The number of phosphatidylserine- and exosome marker-positive EVs did not correlate with urine osmolality and were similar in fresh vs. frozen and between two sequential urine collections from the same individual. Sex differences: Urine from women controls contained greater (P < 0.05) numbers of EVs positive for phosphatidylserine...Continue Reading

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Citations

Jan 16, 2016·BioMed Research International·Lotte Hatting PugholmMalene Møller Jørgensen
Sep 16, 2015·The Journal of Pediatrics·Kirsten KusumiAndrew L Schwaderer
Jul 22, 2017·Journal of the American Society of Nephrology : JASN·Sarwat I GilaniVesna D Garovic
May 13, 2015·Biology of Sex Differences·Callie M GustafsonMuthuvel Jayachandran
Mar 4, 2017·Scientific Reports·Alexia Auffèves, Philippe Grangier
May 29, 2018·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Alexia Auffèves, Philippe Grangier
Aug 23, 2019·Journal of the American Heart Association·Adrian SantelliLilach O Lerman
Aug 9, 2020·International Journal of Molecular Sciences·Félix VargasJoaquín García-Estañ
Feb 23, 2020·Frontiers in Pharmacology·Visith Thongboonkerd
May 18, 2016·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Soon Hyo KwonLilach O Lerman
Jun 21, 2018·Frontiers in Physiology·Gorjana RackovAngel Ayuso-Sacido

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Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
light scattering
light-scattering
urine collection
urine collections

Software Mentioned

JMP

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