Extracellular Vesicles of Alzheimer's Disease Patients as a Biomarker for Disease Progression.

Molecular Neurobiology
Anat AharonJudith Aharon-Peretz

Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative brain pathology and the most common form of dementia. Evidence suggests that extracellular vesicles (EVs) containing cytokines and microRNA are involved in inflammation regulation. The current study aimed to explore a potential impact of AD patients' EVs on disease progression. Blood samples were collected after obtaining signed informed consent (No. 0462-14-RMB) from 42 AD patients at three stages of disease severity and from 19 healthy controls (HC). EV size and concentration were studied by nanotracking analysis. EV membrane antigens were defined by flow cytometry and Western blot; EV protein contents were screened by protein array; the miRNA content was screened by nanostring technology and validated by RT-PCR. HC and AD patients' EVs consisted of a mixture of small (< 100 nm) and larger vesicles. The myelin oligodendrocyte glycoprotein (MOG) expression on EVs correlated with disease severity. EVs of patients with moderate and severe AD had significantly higher levels of MOG, compared with mild AD patients. Levels of EVs expressing the axonal glycoprotein CD171 were significantly higher in severe AD patients than in HC. Increase in endothelial EVs was observed in A...Continue Reading

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Citations

Sep 25, 2020·Journal of Personalized Medicine·Walter J LukiwYuhai Zhao
Mar 3, 2021·International Journal of Rheumatic Diseases·Xiang WangYangang Wang
Feb 23, 2021·Progress in Neurobiology·Yun XiaoXing Li

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Methods Mentioned

BETA
blood collection
flow cytometry
protein array
FACS

Software Mentioned

[UNK] Imager
GraphPad Prism
Total lab
Easy
miRPath
MicroT
MyImageAnalysis
nSolver
GraphPad

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