Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells.

Neurobiology of Disease
Hyung Joon ChoMichal Toborek

Abstract

Human immunodeficiency virus-1 (HIV-1) has been shown to cross the blood-brain barrier and cause HIV-associated neurocognitive disorders (HAND) through a process that may involve direct or indirect interactions with the central nervous system (CNS) cells and alterations of amyloid β (Aβ) homeostasis. The present study focused on the mechanisms of HIV-1 infecting human neural progenitor cells (hNPCs) and affecting NPC intercellular communications with human brain endothelial cells (HBMEC). Despite the lack of the CD4 receptor, hNPCs were effectively infected by HIV-1 via a mechanism involving the chemokine receptors, CXCR4 and CCR5. HIV-1 infection increased expression of connexin-43 (Cx43), phosphorylated Cx43 (pCx43), and pannexin 2 (Panx2) protein levels in hNPCs, suggesting alterations in gap-junction (GJ) and pannexin channel communication. Indeed, a functional GJ assay indicated an increase in communication between HIV-infected hNPCs and non-infected HBMEC. We next analyzed the impact of HBMEC-derived extracellular vesicles (EVs) and EVs carrying Aβ (EV-Aβ) on the expression of Cx43, pCx43, and Panx2 in HIV-1 infected and non-infected hNPCs. Exposure to EV-Aβ resulted in significant reduction of Cx43 and pCx43 protein expr...Continue Reading

References

Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C C BleulC R Mackay
Apr 7, 2000·Brain Research. Brain Research Reviews·R RozentalM F Mehler
Feb 15, 2003·Neurobiology of Disease·Susana Alvarez LosadaMa Angeles Muñoz-Fernández
Sep 25, 2003·Physiological Reviews·Juan C SaezEric C Beyer
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Roberto BruzzoneHannah Monyer
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Paul D Lampe, Alan F Lau
Jun 25, 2004·The Journal of Infectious Diseases·Mitchell D Krathwohl, Jodi L Kaiser
Jun 29, 2004·Journal of Virology·Diane M P LawrenceEugene O Major
Aug 24, 2007·The Journal of Biological Chemistry·Daniela BoassaGina Sosinsky
Nov 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eliseo A Eugenin, Joan W Berman
Dec 20, 2007·AIDS·Ina RothenaignerRuth Brack-Werner
Nov 20, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jiqing Xu, Tsuneya Ikezu
Dec 11, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Bruce J BrewGilles Guillemin
Mar 17, 2009·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Cristian L AchimUNKNOWN Neurobehavioral Research Center
Mar 25, 2009·The Biochemical Journal·Joell L Solan, Paul D Lampe
Feb 19, 2010·The Journal of Biological Chemistry·Marcelo F SantiagoEliana Scemes
Jun 10, 2010·The Journal of Biological Chemistry·Leigh Anne SwayneSteffany A L Bennett
Oct 15, 2010·Nature·Annika ArmulikChrister Betsholtz
Dec 15, 2010·Neurobiology of Disease·Myoung-Hwa LeeArun Venkatesan
Jul 1, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eliseo A EugeninJoan W Berman
Feb 7, 2012·Biochimica Et Biophysica Acta·Silvia PenuelaDale W Laird
Mar 31, 2012·Neural Development·Leigh E Wicki-StordeurLeigh Anne Swayne
Sep 6, 2012·Journal of Cellular and Molecular Medicine·Shinsuke NakagawaMichal Toborek
Oct 19, 2013·Journal of Neurochemistry·Juan Andres OrellanaEliseo Alberto Eugenin
Oct 25, 2013·Frontiers in Pharmacology·Lene N AxelsenMorten S Nielsen
Mar 29, 2014·Frontiers in Physiology·Carola Meier, Katja Rosenkranz
May 23, 2014·Frontiers in Cellular Neuroscience·Paul Castellano, Eliseo A Eugenin
Jun 13, 2014·Frontiers in Cellular Neuroscience·Jessica L WilliamsRobyn S Klein
Oct 10, 2014·ACS Nano·Raul Perez-JimenezJulio M Fernandez
Jun 30, 2015·Cellular and Molecular Life Sciences : CMLS·Elke DecrockLuc Leybaert
Nov 26, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Victor CastroMichal Toborek
Mar 5, 2016·Journal of Neuroinflammation·Joan W BermanEliseo A Eugenin

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.