Extracellular vesicles yield predictive pre-eclampsia biomarkers

Journal of Extracellular Vesicles
Kok Hian TanSai Kiang Lim

Abstract

Circulating extracellular vesicles (EVs) such as cholera toxin B chain (CTB)- or annexin V (AV)-binding EVs were previously shown to be rich sources of biomarkers. Here we test if previously identified pre-eclampsia (PE) candidate biomarkers, TIMP-1 in CTB-EVs (CTB-TIMP) and PAI-1 in AV-EVs (AV-PAI) complement plasma PlGF in predicting PE in a low-risk obstetric population. Eight hundred and forty-three prospectively banked plasma samples collected at 28 + 0 to 32 + 0 gestation weeks in the Neonatal and Obstetrics Risk Assessment (NORA) cohort study were assayed by sandwich ELISAs for plasma PlGF, CTB-TIMP1 and AV-PAI1. Nineteen patients subsequently developed PE 7.3 (±2.9) weeks later at a mean gestational age of 36.1 ± 3.5 weeks. The biomarkers were assessed for their predictive accuracy for PE using stepwise multivariate logistic regression analysis with Firth correction and Areas under the curve (AUC). To achieve 100% sensitivity in predicting PE, the cut-off for plasma PlGF, CTB-TIMP1 & AV-PAI1 were set at <1235, ≤300 or >1300 and <10,550 pg/mL plasma, respectively. The corresponding AUCs, specificity and PPV at a 95% confidence interval were 0.92, 52.1% and 4.7%; 0.72, 44.5% and 4.0%; and 0.69, 21.5% and 2.9%, respectivel...Continue Reading

References

Sep 1, 1996·European Journal of Obstetrics, Gynecology, and Reproductive Biology·M KolbenH Tschesche
Dec 28, 2012·BMC Pregnancy and Childbirth·Wai Yee LimKenneth Y C Kwek
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Dec 29, 2013·Journal of Extracellular Vesicles·Soon Sim TanSai Kiang Lim
Mar 25, 2014·American Journal of Obstetrics and Gynecology·Kok Hian TanSai Kiang Lim
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever
Jul 22, 2015·Journal of Extracellular Vesicles·Richard J LobbAndreas Möller
Jan 7, 2016·The New England Journal of Medicine·Harald ZeislerStefan Verlohren
Mar 2, 2016·Journal of Extracellular Vesicles·Ruenn Chai LaiSai Kiang Lim

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Citations

Feb 15, 2018·Biomarkers in Medicine·Lucia PaoliniAnnalisa Radeghieri
Feb 2, 2018·Endocrine Reviews·Carlos SimonFelipe Vilella
Aug 30, 2018·Journal of Lipid Research·Ruenn Chai Lai, Sai Kiang Lim
Jul 3, 2020·International Journal of Molecular Sciences·Francisca DiasRui Medeiros
Jun 19, 2020·International Journal of Molecular Sciences·Liliana Czernek, Markus Düchler
Jun 16, 2019·International Journal of Molecular Sciences·Barbora KonečnáGabriela Repiská
Jan 21, 2021·Frontiers in Veterinary Science·Bahram TalebjediMarcia De Almeida Monteiro Melo Ferraz
Jan 30, 2021·BioMed Research International·Yuan YaoXinwei Han
Mar 30, 2021·Biology of Reproduction·Zheng WangJinjun Liu
May 29, 2021·Frontiers in Immunology·Kunfeng BaiPhilip C N Chiu

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Methods Mentioned

BETA
ELISA
scanning electron microscopy

Software Mentioned

STATA
SAS

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