PMID: 9528653Apr 7, 1998Paper

Extracellular volume in streptococcal model biofilms: effects of pH, calcium and fluoride

Biochimica Et Biophysica Acta
R K Rose, S J Turner

Abstract

Diffusion, which limits nutrient penetration and end-product export in biofilms, is restricted by reversible binding and extracellular volume fraction (Ve). Fluoride has been demonstrated to prevent calcium bridging, hence inhibiting calcium-mediated cell association (Rose, Lee and Shellis, Caries Res. 30 (1996) 458-464). 3H-inulin effusion measurements from streptococcal model plaques, at pH 7.0 or 5.0, 0-20 mmol/l Ca2+, and with or without 5 mmol/l KF, demonstrated that Ve was greatest in the absence of added Ca2+ and at pH 7.0, lowest at 20 mmol/l Ca2+ and pH 5.0, and that F- raised the minimum Ve. By bridging adjacent cells and reducing the net negative charge, calcium and low pH, respectively, reduce Ve. Fluoride eliminates the calcium-bridging effect, hence increasing Ve.

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Citations

Sep 6, 2003·Applied and Environmental Microbiology·Katharine Kierek, Paula I Watnick
Aug 31, 2010·Journal of Diabetes and Its Complications·Muhammad JawedAbid Azhar
Feb 14, 2006·Journal of Bioscience and Bioengineering·Yoshitaka KawakamiMasashi Nakayama
Apr 28, 2012·Medical Hypotheses·Suzette V van der Waal, Lucas W M van der Sluis
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D G Cvitkovitch
Oct 29, 2004·Journal of Oral Science·Yoshiaki NomuraNobuhiro Hanada

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