PMID: 8589556Jul 1, 1995Paper

Extracorporeal development and ultrarapid freezing of human fetal ova

Journal of Assisted Reproduction and Genetics
J ZhangM DiMattina

Abstract

The present study was performed to culture human fetal ova to determine whether they can be matured and cryopreserved using ultrarapid freezing. Thirty-three pairs of fetal ovaries were obtained from fetuses of 16-20 weeks' gestation following elective abortion. Ovarian tissues were minced into approximately 1-mm sizes and cultured in Waymouth media either before or after ultrarapid freezing. The Waymouth medium was supplemented with 15% (v/v) fetal bovine serum, 0.03 IU/ml FSH and 35 ng/ml insulin. The tissue was cultured at 37 degrees C in 5% CO2 in air for 5-25 days in Falcon dishes and 30-40 days in Costar Transwell-COL membranes prior to induction of final maturation in the presence of LH and human follicular fluid. Minced tissues were also frozen by ultrarapid freezing in M199 with 4.2 M dimethylsulfoxide (DMSO) and 0.35 M sucrose and plunged directly into liquid nitrogen. For thawing, the straws were plunged into a 37 degrees C water bath for 5 s. The contents were then expelled and diluted 1:5 with thawing medium containing 0.42 M sucrose. After washing the thawed tissues were cultured as described for the fresh tissues. Patches of monolayer consisting of fibroblasts had formed within 2-3 days of culture of fresh tissue...Continue Reading

References

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Citations

Mar 26, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Debra A GookC Stern
Mar 26, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Outi Hovatta
Mar 24, 2004·Fertility and Sterility·Tal Biron-ShentalRonit Abir
Jan 13, 2001·Molecular and Cellular Endocrinology·O Hovatta
Apr 25, 2000·Molecular and Cellular Endocrinology·A H SathananthanA Trounson
Jan 1, 1999·Maturitas·J Smitz, R Cortvrindt
May 22, 2003·Best Practice & Research. Clinical Obstetrics & Gynaecology·Outi Hovatta
Jul 9, 2009·Molecular Human Reproduction·G M HartshorneF Ghafari
Nov 22, 2002·Biology of Reproduction·Juergen LiebermannMichael J Tucker
Jun 23, 2005·Reproductive Biomedicine Online·Outi Hovatta
Oct 13, 2007·Theriogenology·J C SadeuJ Smitz
Jun 17, 2011·Reproductive Biomedicine Online·Christiani A AmorimJacques Donnez
Apr 18, 2006·Fertility and Sterility·Jean Clair SadeuJohan Smitz
Dec 22, 1999·Human Reproduction·D A Gook, D H Edgar
Feb 21, 2004·Reproduction, Fertility, and Development·Gohar RahimiFrank Nawroth
Aug 29, 2017·Journal of Adolescent and Young Adult Oncology·Takayuki HainoNao Suzuki

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