Extracorporeal removal of proinflammatory cytokines by specific absorption onto microspheres

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
C Weber, D Falkenhagen

Abstract

Elevated plasma concentrations of interleukin 1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha) derived from cellular stimulation have been found to correlate well with the systemic inflammatory response syndrome and clinical outcome of sepsis. Consequently, biologic inactivation and extracorporeal removal of these potent mediators gain increasing attractiveness as adjunctive therapeutic options. In realization of the latter strategy the authors developed specific adsorbents by covalently linking polyclonal antibodies against IL-1 beta and TNF alpha onto microspheres. The attachment process was characterized by high retention of antigen neutralizing activity. Batch testing of the adsorbents revealed specificity, biocompatibility, and high binding capacity (20.2 and 36.9 ng/mg of particles for IL-1 beta and TNF alpha, respectively). Employment of the particles in the Microspheres Based Detoxification System (MDS) resulted in efficient purification: human plasma spiked with recombinant IL-1 beta and TNF alpha (500 pg/ml) could be cleared at 42 ml/min (IL-1 beta) and 55 ml/min (TNF alpha) at a flow rate of 200 ml/min. These clearance rates are considerably higher than the values obtained with ultrafiltration. In concl...Continue Reading

Citations

May 19, 2010·Biomarkers in Medicine·Sachin S SoniDinna N Cruz
Dec 17, 2010·Annals of Biomedical Engineering·Azadeh Alikhani, William J Federspiel
Jul 26, 2019·The Journal of Physiology·Wilfried Gyselaers, Basky Thilaganathan
Jun 23, 2001·Perfusion·A E Basile-Borgia, V C Ware
Jan 6, 2016·The International Journal of Artificial Organs·Bernd Stegmayr, Horst Klinkmann
Jan 18, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Helmut Borberg

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