Extracorporeal shock waves enhance normal fibroblast proliferation in vitro and activate mRNA expression for TGF-beta1 and for collagen types I and III.

Acta Orthopaedica
Laura BertaRoberto Frairia

Abstract

Extracorporeal shock waves (ESWs) are used to good effect in the treatment of soft tissue injuries, but the underlying mechanisms are still unknown. We therefore determined the effects of ESWs on normal fibroblasts in vitro, in order to assess treatment-induced cell response. A normal human fibroblast cell line (NHDF-12519) was treated with ESWs generated by a piezoelectric device (Piezoson 100; Richard Wolfe) using different protocols of impulses (300, 1,000, or 2,000 shots) and energy (0.11 or 0.22 mJ/mm(2)). Untreated controls and treated cells were cultivated for 12 days following a single shock-wave treatment. Viability, growth rate, and expression of mRNA for TGFbeta-1 and collagen types I and III were evaluated at days 3, 6, 9, and 12. 1 hour after shock-wave treatment, cell viability showed a decrease related mainly to impulse numbers applied. Fibroblasts treated with energy of 0.22 mJ/mm(2) subsequently showed an increase in proliferation from day 6 to day 9 that was higher than in untreated controls, without interference with the normal cell kinetic profile. mRNA expression was also higher in treated fibroblasts than in untreated controls for TGFbeta-1 on day 6 and day 9, for collagen type I on day 6, and for collagen...Continue Reading

References

Mar 24, 2000·International Review of Cytology·M Benjamin, J R Ralphs
Dec 7, 2002·Life Sciences·Sergei P Atamas
Oct 9, 2003·Breast Cancer Research and Treatment·Roberto FrairiaLaura Berta
Dec 16, 2003·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Lucia MartiniRoberto Giardino
Mar 17, 2004·Clinical Orthopaedics and Related Research·Roy K AaronBruce J Simon
Jun 9, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yeung-Jen ChenFeng-Sheng Wang
Jul 14, 2004·The Journal of Hand Surgery·Charles Hsu, James Chang
Dec 21, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Linda A DahlgrenAlan J Nixon
Jan 7, 2005·The Journal of Bone and Joint Surgery. American Volume·Pankaj Sharma, Nicola Maffulli
Feb 8, 2005·Biochemical and Biophysical Research Communications·Jameel Iqbal, Mone Zaidi
Mar 3, 2005·Nitric Oxide : Biology and Chemistry·Sofia MariottoHisanori Suzuki
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Viola Vogel
Apr 26, 2007·British Medical Bulletin·Jan D RompeNicola Maffulli
Sep 22, 2007·The American Journal of Sports Medicine·Lin WangKwok-sui Leung
Jan 4, 2008·The Journal of Bone and Joint Surgery. American Volume·Jan D RompeNicola Maffulli
Jan 29, 2008·Ultrasound in Medicine & Biology·Yuan-Hung ChaoMing-Hong Chen

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Citations

May 28, 2011·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Mario VetranoVincenzo Visco
Nov 23, 2011·The Tohoku Journal of Experimental Medicine·Guang YangYimin Chai
Feb 1, 2013·American Journal of Veterinary Research·Kaitlyn A LinkBrandon N Lillie
Aug 3, 2013·The Bone & Joint Journal·W-H HsuR W-W Hsu
Aug 15, 2015·BioMed Research International·Tobias KischRobert Kraemer
Sep 1, 2017·Knee Surgery & Related Research·Leanne Dupley, Charalambos P Charalambous
Jan 1, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Dick Ho Kiu ChowLing Qin
Aug 30, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Susinder SundaramSandeep M Eswarappa
Nov 5, 2010·The Journal of Bone and Joint Surgery. American Volume·Jan D RompeNicola Maffulli
May 20, 2015·Musculoskeletal Surgery·A NotarnicolaB Moretti
Apr 1, 2016·Journal of Therapeutic Ultrasound·Kristin Dietz-LaursonnMatías de la Fuente
Jan 26, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Letizia RinellaMaria Graziella Catalano
Jan 15, 2021·Advances in Skin & Wound Care·Siqi FuDennis P Orgill
Mar 23, 2021·Clinics in Podiatric Medicine and Surgery·Travis Motley
Sep 4, 2021·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Na JiaQing He

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Methods Mentioned

BETA
FCS
PCR

Software Mentioned

Beacon Designer
SPSS

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