Extract of Azadirachta indica (neem) leaf induces apoptosis in rat oocytes cultured in vitro

Fertility and Sterility
Shail K ChaubeTulsidas G Shrivastav

Abstract

To determine whether aqueous neem leaf extract (NLE) could induce degeneration of rat oocytes and, if so, whether apoptosis is involved during NLE-induced degeneration of oocytes cultured in vitro. A controlled prospective study. Laboratory research setting at Department of Reproductive Biomedicine of the Institute. Fifty-four sexually immature female rats that were 24-25 days of age. The immature female rats were injected with 10 IU pregnant mare serum gonadotropin for 48 h followed by 10 IU human chorionic gonadotropin (hCG) for 16 h. After 16 h, the rats were killed and ovulated cumulus oocyte complexes were collected from the oviduct. Cumulus-enclosed as well as denuded oocytes were used in the present study. Rates of shrinkage, membrane leakage, degeneration, assessment of morphological apoptotic changes, bax protein expression, and DNA fragmentation. The NLE induced morphologic apoptotic changes such as shrinkage, membrane leakage, and cytoplasmic fragmentation prior to degeneration of oocytes. The NLE-treated oocytes that had morphologic apoptotic features showed overexpression of bax protein and DNA fragmentation as evidenced by terminal deoxynucleotidyl transferase nick-end labeling-positive staining and DNA ladder pat...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1993·Life Sciences·S C Juneja, R S Williams
Dec 1, 1993·Immunology Today·L M Schwartz, B A Osborne
Jul 1, 1996·Human Reproduction·Y FujinoM Inoue
Dec 1, 1996·Contraception·S K SharmaW Selvamurthy
May 25, 1999·Molecular Human Reproduction·G I PerezJ L Tilly
Dec 20, 2000·Fertility and Sterility·J WuX Wang
Mar 6, 2003·Vox Sanguinis·Serge Côté
Jun 26, 2003·Journal of Agricultural and Food Chemistry·Vandana SharmaBalraj S Parmar
Aug 12, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P K Khan, K S Awasthy
Oct 17, 2003·Contraception·B Khillare, T G Shrivastav
Dec 31, 2003·Journal of Ethnopharmacology·M Raveendra PaiN Udupa
Feb 10, 2004·Biology of Reproduction·Yanhong HaoRandall S Prather
Mar 17, 2004·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Bina Shaheen SiddiquiSyed Naim-ul-Hassan Naqvi
Apr 7, 2004·Fertility and Sterility·Ralf HenkelThinus F Kruger
May 13, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·I J UdeinyaE N Shu
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Andrea Jurisicova, Beth M Acton
Nov 9, 2004·Toxicon : Official Journal of the International Society on Toxinology·Carolina B WandscheerJosé D Fontana
Mar 22, 2005·Current Medicinal Chemistry. Anti-cancer Agents·R Subapriya, S Nagini
Apr 26, 2005·Journal of Ethnopharmacology·Pongtip SithisarnWandee Gritsanapan
Jun 4, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J K RoopS S Guraya
Sep 1, 2005·Apoptosis : an International Journal on Programmed Cell Death·S K ChaubeT G Shrivastav

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Citations

Feb 9, 2012·Apoptosis : an International Journal on Programmed Cell Death·Anima Tripathi, Shail K Chaube
Dec 25, 2007·Cloning and Stem Cells·Shail K ChaubeTulsidas G Shrivastav
Jun 19, 2013·International Journal of Applied & Basic Medical Research·Anima TripathiShail K Chaube
May 23, 2015·Journal of Biomedical Science·Shilpa PrasadShail K Chaube
May 11, 2015·Apoptosis : an International Journal on Programmed Cell Death·Meenakshi TiwariShail K Chaube
Oct 21, 2018·Journal of Cellular Physiology·Govind R ChaudharyShail K Chaube
Jun 8, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Shashank M PatilNagendra Prasad M N

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