Extracted chitosan disrupts quorum sensing mediated virulence factors in Urinary tract infection causing pathogens

Pathogens and Disease
Durairajan RubiniParamasivam Nithyanand

Abstract

Quorum sensing (QS) plays an important role during the aetiology of urinary tract infection (UTI), as several virulence factors are under the regulation of QS. Pseudomonas aeruginosa and Serratia marcescens, the primary causative agents of UTI, employ acyl homoserine lactone (AHL) as signal molecules to coordinate various virulence factors. In this present study, chitosan extracted from the marine crab Portunus sanguinolentus was screened for its ability to inhibit the QS-signaling molecules of P. aeruginosa (PA01) and few clinical isolates of P. aeruginosa and S. marcescens. The extracted chitosan on comparison with a commercial chitosan showed significant inhibition of several QS-dependent virulence factors in P. aeruginosa and S. marscenes. Furthermore, qPCR analysis was carried out to confirm the down-regulation of fimA, fimC and flhD genes involved in adhesion and pathogenesis of S. marcescens and lasI and rhlI genes that governs the P. aeruginosa quorum sensing system. Moreover, the chitosan when coated on a catheter was also able to disrupt the mature biofilms which was revealed by scanning electron microscopy. Collectively, the present study showcases the QS inhibitory property of extracted chitosan from crab shells whi...Continue Reading

References

Apr 11, 1997·The Journal of Biological Chemistry·E KesslerD E Ohman
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·M R ParsekE P Greenberg
May 21, 1999·Science·J W CostertonE P Greenberg
Dec 2, 2000·International Journal of Medical Microbiology : IJMM·F L Yang, V Braun
Jan 4, 2001·Microbes and Infection·K P RumbaughA N Hamood
Oct 29, 2003·Current Opinion in Chemical Biology·Hiroaki Suga, Kristina M Smith
Nov 11, 2003·Biomacromolecules·Entsar I RabeaWalter Steurbaut
Sep 15, 2004·International Journal of Antimicrobial Agents·Paul A Tambyah
Dec 9, 2004·Chemical Reviews·M N V Ravi KumarA J Domb
Jan 11, 2005·Trends in Microbiology·C A FuxP Stoodley
Apr 12, 2005·Eukaryotic Cell·Gordon RamageJosé L López-Ribot
Nov 23, 2005·Archives of Otolaryngology--head & Neck Surgery·Eric W WangRichard A Chole
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeff G LeidAlyssa K Jeffers
Jan 24, 2007·Journal of Bacteriology·Maurizio LabbateStaffan Kjelleberg
Mar 21, 2007·Applied and Environmental Microbiology·Takenori IshidaJunichi Kato
Aug 7, 2007·Journal of Bacteriology·Hans-Curt FlemmingDaniel J Wozniak
Jan 15, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R Le BerreUNKNOWN Pyopneumagen Group
Jan 29, 2009·International Journal of Biological Sciences·San TinMeena K Sakharkar
Oct 23, 2009·FEMS Immunology and Medical Microbiology·Ramalingam ThenmozhiShunmugiah Karutha Pandian
Dec 19, 2009·Current Microbiology·Paramasivam NithyanandShunmugiah Karutha Pandian
Aug 18, 2010·Infection and Immunity·Chuan-Sheng LinHsin-Chih Lai
Feb 23, 2011·International Journal of Molecular Sciences·Belen OrgazCarmen San Jose
Nov 29, 2013·Scientific Reports·Amin ZhangJinyou Duan
Dec 6, 2014·International Journal of Molecular Sciences·Haibo MuJinyou Duan
Feb 17, 2015·Yonsei Medical Journal·Sun Bean KimJune Myung Kim
May 28, 2015·Pathogens and Disease·Ramesh Salini, Shunmugiah Karutha Pandian
Jul 18, 2015·Microbiology Spectrum·Dominique H LimoliDaniel J Wozniak
Aug 6, 2015·World Journal of Clinical Cases·Israel Castillo-JuárezRodolfo García-Contreras
Sep 29, 2015·Microbiological Research·Paramasivam NithyanandShunmugiah Karutha Pandian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.