Extraction and identification of synovial tissue-derived exosomes by different separation techniques

Journal of Orthopaedic Surgery and Research
Pu ChenQingfu Wang

Abstract

The aim of this study is to compare the efficiency of different separation techniques for extracting synovial tissue-derived exosomes. The synovial tissue discarded during knee arthroscopy or total knee arthroplasty surgery was collected from the Third Affiliated Hospital of Beijing University of Chinese Medicine. Ultracentrifugation (UC), filtration combined with size exclusion chromatography (SECF), and 8% polyethylene glycol (PEG) were used to extract synovial tissue-derived exosomes. Transmission electron microscopy (TEM), nanoparticle tracer analysis (NTA), and Western Blot (WB) were used to detect the morphology, particle size, and biomarker proteins (CD9, CD63, Flotillin-1, and calnexin) of exosomes. The extracts of enriched round and discoid vesicles were successfully extracted with UC, SECF, and PEG. The results of TEM have shown that all three extraction methods can extract circular or elliptical vesicles with disc- and cup-shaped structures from the synovial tissue, with the diameter is about 30-150 nm. NTA suggested the main peaks of three groups of exosomes are around 100-120 nm, and the concentration of the three groups of exosomes was greater than 1 × 1010/ml. The results of WB showed that three positive protein ...Continue Reading

References

Dec 1, 2012·Osteoarthritis and Cartilage·F Berenbaum
Aug 27, 2013·Osteoarthritis and Cartilage·T Neogi
Feb 5, 2014·Nature Reviews. Gastroenterology & Hepatology·Sara LemoinnePierre-Emmanuel Rautou
Dec 10, 2014·Annals of the Rheumatic Diseases·I AtukoralaD J Hunter
Mar 19, 2015·Current Opinion in Rheumatology·Charles J Malemud
Feb 24, 2016·Stem Cells International·John BurkeSadanand Fulzele
Mar 8, 2016·Nature Communications·Defang LiGe Zhang
Sep 17, 2016·Current Rheumatology Reviews·Luana MancarellaRiccardo Meliconi
Nov 2, 2016·Current Protocols in Immunology·Lucia Mincheva-NilssonEva Dehlin
Jan 4, 2018·Journal of the American Society of Nephrology : JASN·Lin-Li LvBi-Cheng Liu
May 16, 2018·Journal of Anatomy·Veronica MacchiRaffaele De Caro
Jul 3, 2018·Journal of Extracellular Vesicles·Andrew D FoersKen C Pang
Nov 2, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Liping WangJing Yu
May 3, 2019·BioMed Research International·Elisa BelluzziVeronica Macchi
Nov 30, 2019·Journal of Orthopaedic Surgery and Research·Pu ChenQingfu Wang

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Methods Mentioned

BETA
transmission electron microscopy
size exclusion chromatography

Software Mentioned

GraphPad Prism

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