PMID: 6167993Jun 1, 1981Paper

Extraction and immunochemical characterization of cholecystokinin-like peptides from pig and rat brain

Proceedings of the National Academy of Sciences of the United States of America
S RyderR S Yalow

Abstract

Two major classes of immunoreactive cholecystokinin peptides (iCCK) have been identified in rat and pig brains: (i) large basic peptides (big iCCK) resembling the 33-amino acid porcine cholecystokinin (pCCK33) in size and charge; (ii) small acidic peptides (small iCCK) resembling the COOH-terminal fragments of CCK. Boiling 0.1 M HCl maximally extracts big iCCK; boiling 0.1 M NaOH maximally extracts small iCCK. The differences in hormonal forms removed by these extractants are not likely to be due to enzymatic conversion during the extraction procedures. Fractionation on Sephadex G-50 and starch gel electrophoresis combined with radioimmunoassay using three antisera of different specificities--(i) directed towards the NH2 terminus of pCCK33, (ii) produced by immunization with COOH-terminal fragment CCK8, (iii) produced by immunization with COOH-terminal fragment CCK4--are consistent with the hypothesis that a major fraction of big iCCK may represent intact cholecystokinin with a COOH-terminal extension, as has recently been suggested for gastrin, a molecule having a COOH-terminal pentapeptide identical with that of cholecystokinin.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B E NoyesK L Agarwal
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G Suchanek, G Kreil
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E MullerR S Yalow
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P RobberechtJ J Vanderhaeghen
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Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E Straus, R S Yalow
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May 30, 1980·Biochemical and Biophysical Research Communications·S W RyderR S Yalow

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Citations

Jun 1, 1990·Brain Research. Developmental Brain Research·N W MogensenJ F Rehfeld
Jan 1, 1981·Peptides·E StrausR S Yalow
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J EngR S Yalow
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R J DeschenesJ E Dixon
Mar 27, 1992·Brain Research·J F RehfeldH J Monstein
Oct 1, 1983·Analytical Biochemistry·F Nyberg, L Terenius
Oct 1, 1983·Neuropeptides·M C Beinfeld
Jan 1, 1985·Annals of the New York Academy of Sciences·J F RehfeldK Stengaard-Pedersen
Jan 27, 1983·Nature·D K Meyer, J Krauss
Jan 1, 1985·Journal of Neurochemistry·J F Rehfeld
Jun 1, 1984·Journal of Neurochemistry·P D Marley, J F Rehfeld
Sep 1, 1984·Journal of Neurochemistry·L J MillerD Speicher
Jan 1, 1983·Journal of Neuroscience Research·P M Simon-AssmannJ C Thompson
Jan 1, 1985·Annals of the New York Academy of Sciences·R J DeschenesJ E Dixon
Jan 1, 1985·Annals of the New York Academy of Sciences·M C Beinfeld, A Ciarleglio

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