Extraction, characterization and immunomodulatory property of pectic polysaccharide from pomegranate peels: Enzymatic vs conventional approach

International Journal of Biological Macromolecules
Hassan Ahmadi GavlighiMaryam Ghaderi-Ghahfarokhi

Abstract

Molecular characteristics, structural properties and immunostimulatory activities of polysaccharides from pomegranate peel were evaluated after 0.1 M HCl, Cellic CTec2 and buffer extractions. The isolated polysaccharides were mainly formed of neutral sugars (32.1%-51.1%) and uronic acids (19.9%-30.8%) as well as varying levels of proteins (15.0%-39.5%). Different levels of sugars including glucose (44.9%-68.1%), galactose (14.6%-19.4%), mannose (3.4%-18.1%), arabinose (3.1%-18.1%) and rhamnose (3.5-6.0%) constructed the structure of isolated polysaccharides. The average molecular weight (Mw) of polysaccharides differed notably and ranged from 422.5 × 103 to 18,631.8 × 103 g/mol. Polysaccharide molecules obtained using Cellic CTec2 enzyme induced RAW264.7 murine macrophage cells to release considerable amount of inflammatory mediators including nitric oxide, IL-1β, TNF-α, IL-6 and IL-10. The polysaccharide stimulation of macrophage cells initiated through NF-κB and MAPKs signaling pathways and activation of p-NF-κB, p-JNK, p-ERK and p-p38 proteins. The most potent immunostimulatory polysaccharides were consisted of (1 → 3)-linked glucose, (1 → 6)-linked galactose, (1 → 4)-linked mannose and (1 → 4)-linked arabinose residues with...Continue Reading

Citations

Jan 26, 2020·Foods·Panagiotis Kandylis, Evangelos Kokkinomagoulos
Jun 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Patricia GullónJosé M Lorenzo
Jun 6, 2019·Frontiers in Plant Science·Irit Bar-Ya'akovDoron Holland
Aug 10, 2021·Frontiers in Physiology·Karien Sauruk da SilvaDaniele Maria-Ferreira

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